Cystatin SA Recombinant Protein | CST2 recombinant protein

Cystatin SA Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120857

• Purity: >98% as determined by SDS-PAGE
• Synonyms: Cystatin SA; Cystatin SA Protein; Human; Recombinant (His Tag); Human Cystatin SA/CST2 Protein (His Tag); CST2 Protein; MGC71924 Protein; cystatin SA; CST2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala141

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Trp 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CST2 (NP_001313.1)(Met 1-Ala 141) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CST2 recombinant protein

Background: Cystatin-SA, also known as Cystatin-2, Cystatin-S5 and CST2, is a secreted protein which belongs to thecystatin family. Cystatin-2/CST2 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). It is also expressed in submandibular gland and parotid gland. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The CST1, CST2, CST4, and CST5 are expressed in differential, tissue-specific patterns. Expression of CST2 and CST5 is restricted to the submandibular and parotid glands, while CST1 and CST4 are expressed in these tissues and in the lacrimal gland.

References

Saitoh E., et al.,(1987), Human cysteine-proteinase inhibitors: nucleotide sequence analysis of three members of the cystatin gene family. Gene 61:329-338.Zhang Z., et al., (2004), Signal peptide prediction based on analysis of experimentally verified cleavage sites.Protein Sci. 13:2819-2824.Deloukas P., et al.,(2001), The DNA sequence and comparative analysis of human chromosome 20.Nature 414:865-871.

NCBI and Uniprot Product Information

• NCBI GI #: 4503105
• NCBI GeneID: 1470
• NCBI Accession #: NP_001313.1
• NCBI GenBank Nucleotide #: NM_001322.2
• UniProt Accession #: P09228; Q9UCQ7
• Molecular Weight: 16,445 Da
• NCBI Official Full Name: cystatin-SA
• NCBI Official Synonym Full Names: cystatin SA
• NCBI Official Symbol: CST2
• NCBI Protein Information: cystatin-SA; cystatin 2; cystatin-2; cystatin S5; cystatin-S5; cysteine-proteinase inhibitor; salivary cysteine (thiol) protease inhibitor
• UniProt Protein Name: Cystatin-SA
• Protein Family: Cystatin
• UniProt Gene Name: CST2
• UniProt Entry Name: CYTT_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a secreted thiol protease inhibitor found at high levels in saliva, tears and seminal plasma. [provided by RefSeq, Jul 2008]

Uniprot Description

CST2: Thiol protease inhibitor. Belongs to the cystatin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: extracellular space

Molecular Function: cysteine protease inhibitor activity

Biological Process: detection of chemical stimulus involved in sensory perception of bitter taste