IMPACT cdna clone
IMPACT
Synonyms
IMPACT; RWDD5; IMPACT cdna clone
Form/Format
Lyophilized
Sequence
Nucleotide Sequence: ATGGCTGAGGGGGACGCAGGGAGCGACCAGAGGCAGAATGAGGAAATTGAAGCAATGGCAGCCATTTATGGCGAGGAGTGGTGTGTCATTGATGACTGTGCCAAAATATTTTGTATTAGAATTAGCGACGATATAGATGACCCCAAATGGACACTTTGCTTGCAGGTGATGCTGCCGAATGAATACCCAGGTACAGCTCCACCTATCTACCAGTTGAATGCTCCTTGGCTTAAAGGGCAAGAACGTGCGGATTTATCAAATAGCCTTGAGGAAATATATATTCAGAATATCGGTGAAAGTATTCTTTACCTGTGGGTGGAGAAAATAAGAGATGTTCTTATACAAAAATCTCAGATGACAGAACCAGGCCCAGATGTAAAGAAGAAAACTGAAGAGGAAGATGTTGAATGTGAAGATGATCTCATTTTAGCATGTCAGCCGGAAAGTTCGGTTAAAGCATTGGATTTTGATATCAGTGAAACTCGGACAGAAGTAGAAGTAGAAGAATTACCTCCGATTGATCATGGCATTCCTATTACAGACCGAAGAAGTACTTTTCAGGCACACTTGGCTCCAGTGGTTTGTCCCAAACAGGTGAAAATGGTTCTTTCCAAATTGTATGAGAATAAGAAAATAGCTAGTGCCACCCACAACATCTATGCCTACAGAATATATTGTGAGGATAAACAGACCTTCTTACAGGATTGTGAGGATGATGGGGAAACAGCAGCTGGTGGGCGTCTTCTTCATCTCATGGAGATTTTGAATGTGAAGAATGTCATGGTGGTAGTATCACGCTGGTATGGAGGGATTCTGCTAGGACCAGATCGCTTTAAACATATCAACAACTGTGCCAGAAACATACTAGTGGAAAAGAACTACACAAATTCACCTGAGGAGTCATCTAAGGCTTTGGGAAAGAACAAAAAAGTAAGAAAAGACAAGAAGAGGAATGAACATTAA
Translation Sequence: MAEGDAGSDQ RQNEEIEAMA AIYGEEWCVI DDCAKIFCIR ISDDIDDPKW TLCLQVMLPN EYPGTAPPIY QLNAPWLKGQ ERADLSNSLE EIYIQNIGES ILYLWVEKIR DVLIQKSQMT EPGPDVKKKT EEEDVECEDD LILACQPESS VKALDFDISE TRTEVEVEEL PPIDHGIPIT DRRSTFQAHL APVVCPKQVK MVLSKLYENK KIASATHNIY AYRIYCEDKQ TFLQDCEDDG ETAAGGRLLH LMEILNVKNV MVVVSRWYGG ILLGPDRFKH INNCARNILV EKNYTNSPEE SSKALGKNKK VRKDKKRNEH
Translation Sequence: MAEGDAGSDQ RQNEEIEAMA AIYGEEWCVI DDCAKIFCIR ISDDIDDPKW TLCLQVMLPN EYPGTAPPIY QLNAPWLKGQ ERADLSNSLE EIYIQNIGES ILYLWVEKIR DVLIQKSQMT EPGPDVKKKT EEEDVECEDD LILACQPESS VKALDFDISE TRTEVEVEEL PPIDHGIPIT DRRSTFQAHL APVVCPKQVK MVLSKLYENK KIASATHNIY AYRIYCEDKQ TFLQDCEDDG ETAAGGRLLH LMEILNVKNV MVVVSRWYGG ILLGPDRFKH INNCARNILV EKNYTNSPEE SSKALGKNKK VRKDKKRNEH
Sequence Length
320
Species
Human
Chromosome Location
18q11.2-q12.1
OMIM Reference Number
615319
cDNA Size
963bp
Vector Description
This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
Vector
(puc19-derived cloning vector)
Preparation Before Usage
1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
Preparation and Storage
Store the plasmid at -20 degree C.
Related Product Information for IMPACT cdna clone
IMPACT belongs to the IMPACT family and contains 1 RWD domain. It is translational regulator that ensures constant high levels of translation under amino acid starvation and acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis.
Product Categories/Family for IMPACT cdna clone
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein IMPACT
UniProt Protein Name
Protein IMPACT
Protein Family
UniProt Gene Name
IMPACT
UniProt Entry Name
IMPCT_HUMAN
Uniprot Description
IMPACT: Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. Belongs to the IMPACT family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 18q11.2-q12.1
Cellular Component: cytoplasm
Biological Process: negative regulation of protein amino acid phosphorylation; regulation of translational initiation
Similar Products
Product Notes
The IMPACT impact (Catalog #AAA200967) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Nucleotide Sequence: ATGGCTGAGG GGGACGCAGG GAGCGACCAG AGGCAGAATG AGGAAATTGA AGCAATGGCA GCCATTTATG GCGAGGAGTG GTGTGTCATT GATGACTGTG CCAAAATATT TTGTATTAGA ATTAGCGACG ATATAGATGA CCCCAAATGG ACACTTTGCT TGCAGGTGAT GCTGCCGAAT GAATACCCAG GTACAGCTCC ACCTATCTAC CAGTTGAATG CTCCTTGGCT TAAAGGGCAA GAACGTGCGG ATTTATCAAA TAGCCTTGAG GAAATATATA TTCAGAATAT CGGTGAAAGT ATTCTTTACC TGTGGGTGGA GAAAATAAGA GATGTTCTTA TACAAAAATC TCAGATGACA GAACCAGGCC CAGATGTAAA GAAGAAAACT GAAGAGGAAG ATGTTGAATG TGAAGATGAT CTCATTTTAG CATGTCAGCC GGAAAGTTCG GTTAAAGCAT TGGATTTTGA TATCAGTGAA ACTCGGACAG AAGTAGAAGT AGAAGAATTA CCTCCGATTG ATCATGGCAT TCCTATTACA GACCGAAGAA GTACTTTTCA GGCACACTTG GCTCCAGTGG TTTGTCCCAA ACAGGTGAAA ATGGTTCTTT CCAAATTGTA TGAGAATAAG AAAATAGCTA GTGCCACCCA CAACATCTAT GCCTACAGAA TATATTGTGA GGATAAACAG ACCTTCTTAC AGGATTGTGA GGATGATGGG GAAACAGCAG CTGGTGGGCG TCTTCTTCAT CTCATGGAGA TTTTGAATGT GAAGAATGTC ATGGTGGTAG TATCACGCTG GTATGGAGGG ATTCTGCTAG GACCAGATCG CTTTAAACAT ATCAACAACT GTGCCAGAAA CATACTAGTG GAAAAGAACT ACACAAATTC ACCTGAGGAG TCATCTAAGG CTTTGGGAAA GAACAAAAAA GTAAGAAAAG ACAAGAAGAG GAATGAACAT TAATranslati on Sequence: MAEGDAGSDQ RQNEEIEAMA AIYGEEWCVI DDCAKIFCIR ISDDIDDPKW TLCLQVMLPN EYPGTAPPIY QLNAPWLKGQ ERADLSNSLE EIYIQNIGES ILYLWVEKIR DVLIQKSQMT EPGPDVKKKT EEEDVECEDD LILACQPESS VKALDFDISE TRTEVEVEEL PPIDHGIPIT DRRSTFQAHL APVVCPKQVK MVLSKLYENK KIASATHNIY AYRIYCEDKQ TFLQDCEDDG ETAAGGRLLH LMEILNVKNV MVVVSRWYGG ILLGPDRFKH INNCARNILV EKNYTNSPEE SSKALGKNKK VRKDKKRNEH. It is sometimes possible for the material contained within the vial of "IMPACT, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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