Western Blot (WB)
(IMPACT Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IMPACT antibody detected the IMPACT protein (arrow).)
Rabbit anti-Human IMPACT Polyclonal Antibody
IMPACT, NT (IMPACT, Protein IMPACT, Imprinted and ancient gene protein homolog)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
IMPACT; Polyclonal Antibody; NT (IMPACT; Protein IMPACT; Imprinted and ancient gene protein homolog); Anti -IMPACT; anti-IMPACT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-IMPACT antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
IMPACT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human IMPACT.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(IMPACT Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IMPACT antibody detected the IMPACT protein (arrow).)
Western Blot (WB)
(IMPACT Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IMPACT antibody detected the IMPACT protein (arrow).)
Related Product Information for anti-IMPACT antibody
Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis (By similarity).
Product Categories/Family for anti-IMPACT antibody
NCBI and Uniprot Product Information
Uniprot Description
IMPACT: Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. Belongs to the IMPACT family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 18q11.2-q12.1
Cellular Component: cytoplasm
Biological Process: negative regulation of protein amino acid phosphorylation; regulation of translational initiation
Similar Products
Product Notes
The IMPACT (Catalog #AAA643408) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IMPACT, NT (IMPACT, Protein IMPACT, Imprinted and ancient gene protein homolog) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IMPACT can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the IMPACT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IMPACT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
