Rabbit anti-Human IMPACT Polyclonal Antibody | anti-IMPACT antibody

IMPACT Antibody

Cat. Num. AAA7004009

• Gene Names: IMPACT; RWDD5
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%,Protein G purified
• Synonyms: IMPACT; Polyclonal Antibody; IMPACT Antibody; Protein IMPACT; Imprinted and ancient gene protein homolog; anti-IMPACT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%,Protein G purified
• Form/Format: Liquid
• Sequence Length: 320

• Applicable Applications for anti-IMPACT antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Recombinant human Protein IMPACT protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: IMPACT antibody at 10 ug/mlLane 1: Hela whole cell lysateLane 2: HL60 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 37,23 kDaObserved band size: 36,52 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human endometrial cancer using MBS7004009 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7004009 at dilution of 1:100)

Related Product Information for anti-IMPACT antibody

Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its action on GCN2, may also facilitate neuritogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 8923819
• NCBI GeneID: 55364
• NCBI Accession #: NP_060909.1
• NCBI GenBank Nucleotide #: NM_018439.3
• UniProt Accession #: Q9P2X3; Q49AM0; Q9H2X4; A8MXG0
• Molecular Weight: 22,999 Da
• NCBI Official Full Name: protein IMPACT
• NCBI Official Synonym Full Names: impact RWD domain protein
• NCBI Official Symbol: IMPACT
• NCBI Official Synonym Symbols: RWDD5
• NCBI Protein Information: protein IMPACT
• UniProt Protein Name: Protein IMPACT
• Protein Family: IMPACT family
• UniProt Gene Name: IMPACT
• UniProt Entry Name: IMPCT_HUMAN

Uniprot Description

IMPACT: Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. Belongs to the IMPACT family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 18q11.2-q12.1

Cellular Component: cytoplasm; polysome

Biological Process: cellular response to amino acid starvation; cellular response to glucose starvation; negative regulation of protein amino acid autophosphorylation; negative regulation of protein amino acid phosphorylation; negative regulation of protein complex assembly; negative regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation