Western Blot (WB)
(IMPACT Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IMPACT antibody detected the IMPACT protein (arrow).)
Rabbit IMPACT Polyclonal Antibody | anti-IMPACT antibody
IMPACT Antibody (N-term)
Gene Names
IMPACT; RWDD5
Reactivity
Human (Predicted Reactivity: Rabbit)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
IMPACT; Polyclonal Antibody; IMPACT Antibody (N-term); Protein IMPACT; Imprinted and ancient gene protein homolog; anti-IMPACT antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Rabbit)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This IMPACT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human IMPACT.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
1-30
Sequence Length
202
Applicable Applications for anti-IMPACT antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(IMPACT Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IMPACT antibody detected the IMPACT protein (arrow).)
Western Blot (WB)
(IMPACT Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IMPACT antibody detected the IMPACT protein (arrow).)
Related Product Information for anti-IMPACT antibody
Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis (By similarity).
References
Habibi, D., et al. J. Cell. Physiol. 225(1):196-205(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Pereira, C.M., et al. J. Biol. Chem. 280(31):28316-28323(2005)
Okamura, K., et al. Biochem. Biophys. Res. Commun. 329(3):824-830(2005)
Okamura, K., et al. DNA Res. 11(6):381-390(2004)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Pereira, C.M., et al. J. Biol. Chem. 280(31):28316-28323(2005)
Okamura, K., et al. Biochem. Biophys. Res. Commun. 329(3):824-830(2005)
Okamura, K., et al. DNA Res. 11(6):381-390(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36476
NCBI Official Full Name
protein IMPACT
NCBI Official Synonym Full Names
impact RWD domain protein
NCBI Official Symbol
IMPACT
NCBI Official Synonym Symbols
RWDD5
NCBI Protein Information
protein IMPACT
UniProt Protein Name
Protein IMPACT
Protein Family
UniProt Gene Name
IMPACT
UniProt Entry Name
IMPCT_HUMAN
Uniprot Description
IMPACT: Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. Belongs to the IMPACT family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 18q11.2-q12.1
Cellular Component: cytoplasm
Biological Process: negative regulation of protein amino acid phosphorylation; regulation of translational initiation
Research Articles on IMPACT
Similar Products
Product Notes
The IMPACT impact (Catalog #AAA9212255) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The IMPACT Antibody (N-term) reacts with Human (Predicted Reactivity: Rabbit) and may cross-react with other species as described in the data sheet. AAA Biotech's IMPACT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the IMPACT impact for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IMPACT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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