Rabbit anti-Human IMPACT Polyclonal Antibody | anti-IMPACT antibody

IMPACT, NT (IMPACT, Protein IMPACT, Imprinted and ancient gene protein homolog) (Azide free) (HRP)

Cat. Num. AAA6310425

• Gene Names: IMPACT; RWDD5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IMPACT; Polyclonal Antibody; NT (IMPACT; Protein IMPACT; Imprinted and ancient gene protein homolog) (Azide free) (HRP); Imprinted and ancient gene protein homolog; anti-IMPACT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-IMPACT antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: IMPACT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human IMPACT.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(IMPACT Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IMPACT antibody detected the IMPACT protein (arrow).)

Related Product Information for anti-IMPACT antibody

Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis (By similarity).

Product Categories/Family for anti-IMPACT antibody

Antibodies; Proteins

References

Habibi, D., et al. J. Cell. Physiol. 225(1):196-205(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Pereira, C.M., et al. J. Biol. Chem. 280(31):28316-28323(2005). Okamura, K., et al. Biochem. Biophys. Res. Commun. 329(3):824-830(2005). Okamura, K., et al. DNA Res. 11(6):381-390(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 8923819
• NCBI GeneID: 55364
• NCBI Accession #: NP_060909.1
• NCBI GenBank Nucleotide #: NM_018439.3
• UniProt Accession #: Q9P2X3; Q49AM0; Q9H2X4; A8MXG0
• Molecular Weight: 22,999 Da
• NCBI Official Full Name: protein IMPACT
• NCBI Official Synonym Full Names: impact RWD domain protein
• NCBI Official Symbol: IMPACT
• NCBI Official Synonym Symbols: RWDD5
• NCBI Protein Information: protein IMPACT; Impact homolog; RWD domain containing 5; imprinted and ancient gene protein homolog
• UniProt Protein Name: Protein IMPACT
• Protein Family: IMPACT family
• UniProt Gene Name: IMPACT
• UniProt Entry Name: IMPCT_HUMAN

Uniprot Description

IMPACT: Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. Belongs to the IMPACT family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 18q11.2-q12.1

Cellular Component: cytoplasm

Biological Process: negative regulation of protein amino acid phosphorylation; regulation of translational initiation