Goat GNAT3 Polyclonal Antibody | anti-GNAT3 antibody

GNAT3 (Guanine Nucleotide-binding Protein G(t) Subunit alpha-3, Gustducin alpha-3 Chain)

Cat. Num. AAA6005782

• Gene Names: GNAT3; GDCA
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: GNAT3; Polyclonal Antibody; GNAT3 (Guanine Nucleotide-binding Protein G(t) Subunit alpha-3; Gustducin alpha-3 Chain); guanine nucleotide binding protein; alpha transducing 3; tcag7.1053; GDCA; gustducin alpha-3; anti-GNAT3 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GNAT3.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-GNAT3 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000.; Western blot: Preliminary experiments gave an approx 27kDa band in Human Colon and Testis lysates and in cell lines NIH 3T3, A431 and Kelly after 0.1?g/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.4kDa according to NP_001095856.1. The 27kDa band was successfully blocked by incubation with the immunizing peptide.; IHC: In paraffin embedded Human Small Intestine shows staining of endocrine cells amongst the enterocytes. Recommended concentration: 3-6?g/ml.
• Immunogen: Peptide with sequence C-KNQFLDLNLKKEDKE, from the internal region of the protein sequence according to NP_001095856.1.
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin embedded human small intestine using MBS6005782 (3.8ug/ml) and AP-staining after steamed antigen retrieval with citrate buffer pH 6. AP Staining.)

Product Categories/Family for anti-GNAT3 antibody

Antibodies; Abs to G Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 156139155
• NCBI GeneID: 346562
• NCBI Accession #: NP_001095856.1
• NCBI GenBank Nucleotide #: NM_001102386.1
• UniProt Accession #: A8MTJ3; A4D1B2; A4D1B3; B9EJG5
• NCBI Official Full Name: guanine nucleotide-binding protein G(t) subunit alpha-3
• NCBI Official Synonym Full Names: guanine nucleotide binding protein, alpha transducing 3
• NCBI Official Symbol: GNAT3
• NCBI Official Synonym Symbols: GDCA
• NCBI Protein Information: guanine nucleotide-binding protein G(t) subunit alpha-3; guanine nucleotide-binding protein G(t) subunit alpha-3; gustducin alpha-3 chain
• UniProt Protein Name: Guanine nucleotide-binding protein G(t) subunit alpha-3
• UniProt Gene Name: GNAT3
• UniProt Entry Name: GNAT3_HUMAN

Uniprot Description

G-alpha t3: Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide- 1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity. Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Protein type: G protein, heterotrimeric; G protein, heterotrimeric alpha G((i/o/t/z)); G protein

Chromosomal Location of Human Ortholog: 7q21.11

Cellular Component: photoreceptor inner segment; protein complex; photoreceptor outer segment; apical plasma membrane; plasma membrane; acrosome; heterotrimeric G-protein complex; axoneme

Molecular Function: GTPase activity; GTP binding; G-protein-coupled receptor binding; metal ion binding; G-protein beta/gamma-subunit binding; G-protein coupled photoreceptor activity

Biological Process: response to nicotine; G-protein signaling, coupled to cAMP nucleotide second messenger; synaptic transmission; platelet activation; sensory perception of umami taste; sensory perception of sweet taste; metabolic process; G-protein signaling, adenylate cyclase inhibiting pathway; detection of chemical stimulus involved in sensory perception of bitter taste; blood coagulation; detection of visible light