CD137 Recombinant Protein | TNFRSF9 recombinant protein

CD137 Protein, Mouse, Recombinant (soluble form, His Tag)

Cat. Num. AAA8122997

• Gene Names: Tnfrsf9; ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD137; CD137 Protein; Mouse; Recombinant (soluble form; His Tag); Mouse TNFRSF9/CD137/4-1BB Protein (soluble form; 4-1BB Protein; A930040I11Rik Protein; AA408498 Protein; AI325004 Protein; Cd137 Protein; CDw137 Protein; ILA Protein; Ly63 Protein; tumor necrosis factor receptor superfamily; member 9; TNFRSF9 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu211

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Val 24
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse Tnfrsf9 (NP_001070976.1)(Met1-Leu211) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TNFRSF9 recombinant protein

Background: CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4⁺ and CD⁸⁺ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor-associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials. Soluble forms of CD137 (sCD137) are generated by differential splicing. sCD137 can bind to CD137 ligand to antagonize the costimulatory activities of the membrane-bound CD137 and reduce T cell proliferation and IL-2 secretion.

Product Categories/Family for TNFRSF9 recombinant protein

Cancer Drug Targets

References

Sica G, et al. (1999) Biochemical and immunological characteristics of 4-1BB (CD137) receptor and ligand and potential applications in cancer therapy. Arch Immunol Ther Exp (Warsz). 47(5): 275-9.Nam KO, et al. (2005) The therapeutic potential of 4-1BB (CD137) in cancer. Curr Cancer Drug Targets. 5(5): 357-63.Wang Q, et al. (2008) Analysis of CD137 and CD137L expression in human primary tumor tissues. Croat Med J. 49(2): 192-200.Melero I, et al. (2008) Multi-layered action mechanisms of CD137 (4-1BB)-targeted immunotherapies. Trends Pharmacol Sci. 29(8): 383-90.Thum E, et al. (2009) CD137, implications in immunity and potential for therapy. Front Biosci. 14: 4173-88.

NCBI and Uniprot Product Information

• NCBI GI #: 117422435
• NCBI GeneID: 21942
• NCBI Accession #: NP_001070976.1
• NCBI GenBank Nucleotide #: NM_001077508.1
• Molecular Weight: 27,598 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 9 isoform 2
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 9
• NCBI Official Symbol: Tnfrsf9
• NCBI Official Synonym Symbols: ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 9
• Protein Family: Tumor necrosis factor receptor superfamily