Typical Testing Data/Standard Curve (for reference only)
Human CoQ10B (Coenzyme Q10 Homolog B) ELISA Kit | CoQ10B elisa kit
Human CoQ10B (Coenzyme Q10 Homolog B) ELISA Kit
Reactivity
Human
Synonyms
CoQ10B (Coenzyme Q10 Homolog B); Human CoQ10B (Coenzyme Q10 Homolog B) ELISA Kit; CoQ10B elisa kit
Reactivity
Human
Specificity
This kit recognizes Human CoQ10B in samples. No significant cross-reactivity or interference between Human CoQ10B and analogues was observed.
Sequence Length
255
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.63-40ng/mL
Sensitivity
0.38ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CoQ10B were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CoQ10B were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CoQ10B elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human CoQ10B concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CoQ10B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CoQ10B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CoQ10B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CoQ10B. You can calculate the concentration of Human CoQ10B in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CoQ10B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CoQ10B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CoQ10B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CoQ10B. You can calculate the concentration of Human CoQ10B in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
24,460 Da
NCBI Official Full Name
coenzyme Q10 homolog A (yeast), isoform CRA_c, partial
NCBI Official Synonym Full Names
coenzyme Q10A
NCBI Official Symbol
COQ10A
NCBI Protein Information
coenzyme Q-binding protein COQ10 homolog A, mitochondrial
UniProt Protein Name
Coenzyme Q-binding protein COQ10 homolog A, mitochondrial
Protein Family
UniProt Gene Name
COQ10A
UniProt Entry Name
CQ10A_HUMAN
Uniprot Description
COQ10A: Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes (Probable). Belongs to the COQ10 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: mitochondrial inner membrane
Research Articles on CoQ10B
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Product Notes
The Human CoQ10B coq10a (Catalog #AAA2533439) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533439 ELISA Kit recognizes Human CoQ10B. It is sometimes possible for the material contained within the vial of "CoQ10B (Coenzyme Q10 Homolog B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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