Western Blot (WB)
(GCC2 Antibody (C-term) western blot analysis in mouse stomach tissue lysates (15ug/lane).This demonstrates the GCC2 antibody detected GCC2 protein (arrow).)
Rabbit anti-Human, Mouse GCC2 Polyclonal Antibody | anti-GCC2 antibody
GCC2, CT (GCC2, KIAA0336, RANBP2L4, GRIP and coiled-coil domain-containing protein 2, 185kD Golgi coiled-coil protein, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, Ran-binding protein 2-like 4, Renal carcinoma antigen NY-REN-53) (AP)
Gene Names
GCC2; REN53; GCC185; RANBP2L4
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
GCC2; Polyclonal Antibody; CT (GCC2; KIAA0336; RANBP2L4; GRIP and coiled-coil domain-containing protein 2; 185kD Golgi coiled-coil protein; CLL-associated antigen KW-11; CTCL tumor antigen se1-1; Ran-binding protein 2-like 4; Renal carcinoma antigen NY-REN-53) (AP); Renal carcinoma antigen NY-REN-53; anti-GCC2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-GCC2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
GCC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1548-1617 amino acids from the C-terminal region of human GCC2.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(GCC2 Antibody (C-term) western blot analysis in mouse stomach tissue lysates (15ug/lane).This demonstrates the GCC2 antibody detected GCC2 protein (arrow).)
Western Blot (WB)
(GCC2 Antibody (C-term) western blot analysis in mouse stomach tissue lysates (15ug/lane).This demonstrates the GCC2 antibody detected GCC2 protein (arrow).)
Immunohistochemistry (IHC)
(GCC2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GCC2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GCC2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GCC2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-GCC2 antibody
The protein encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-GCC2 antibody
References
Houghton, F.J., et al. Cell 138(4):787-794(2009). Hayes, G.L., et al. Mol. Biol. Cell 20(1):209-217(2009). Burguete, A.S., et al. Cell 132(2):286-298(2008). Efimov, A., et al. Dev. Cell 12(6):917-930(2007). Derby, M.C., et al. Traffic 8(6):758-773(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
5,043 Da
NCBI Official Full Name
GRIP and coiled-coil domain-containing protein 2
NCBI Official Synonym Full Names
GRIP and coiled-coil domain containing 2
NCBI Official Symbol
GCC2
NCBI Official Synonym Symbols
REN53; GCC185; RANBP2L4
NCBI Protein Information
GRIP and coiled-coil domain-containing protein 2
UniProt Protein Name
GRIP and coiled-coil domain-containing protein 2
UniProt Gene Name
GCC2
UniProt Synonym Gene Names
KIAA0336; RANBP2L4; GCC185; RanBP2L4
NCBI Description
The protein encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
Uniprot Description
Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector it is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. May also play a role in transport between the recycling endosomes and the Golgi. Required for maintenance of the Golgi structure, it is involved in the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2.
Research Articles on GCC2
Similar Products
Product Notes
The GCC2 gcc2 (Catalog #AAA6301555) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GCC2, CT (GCC2, KIAA0336, RANBP2L4, GRIP and coiled-coil domain-containing protein 2, 185kD Golgi coiled-coil protein, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, Ran-binding protein 2-like 4, Renal carcinoma antigen NY-REN-53) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GCC2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GCC2 gcc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
