RNF7 recombinant protein

RNF7, 1-113aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205480

• Gene Names: RNF7; SAG; ROC2; CKBBP1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: RNF7; 1-113aa; Human; His tag; E Coli (Denatured); RING-box protein 2 isoform 1; Ring finger protein 7; CKBBP1; ROC2; SAG; RNF7 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 0.4M urea
• Concentration: 1.0 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADVEDG EETCALASHS GSSGSKSGGD KMFSLKKWNA VAMWSWDVEC DTCAICRVQV MDACLRCQAE NKQEDCVVVW GECNHSFHNC CMSLWVKQNN RCPLCQQDWV VQRIGK
• Sequence Length: 113

• Applicable Applications for RNF7 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RNF7 recombinant protein

RNF7 is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHuK/IKK-alpha/IKBKA) and p27Kip1 (CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported. Recombinant human RNF7 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for RNF7 recombinant protein

Metabolism

References

Swaroop M., et al. (2000). Oncogene 19:2855-2866

NCBI and Uniprot Product Information

• NCBI GI #: 7657522
• NCBI GeneID: 9616
• NCBI Accession #: NP_055060
• NCBI GenBank Nucleotide #: NM_014245.4
• UniProt Accession #: Q9UBF6; Q9BXN8; Q9Y5M7; A8K1H9; A8MTB5; C9JYL3; D3DNF7; D3DNF8
• Molecular Weight: 15.1 kDa (136aa)
• NCBI Official Full Name: RING-box protein 2 isoform 1
• NCBI Official Synonym Full Names: ring finger protein 7
• NCBI Official Symbol: RNF7
• NCBI Official Synonym Symbols: SAG; ROC2; CKBBP1
• NCBI Protein Information: RING-box protein 2
• UniProt Protein Name: RING-box protein 2
• Protein Family: RING-box protein
• UniProt Gene Name: RNF7
• UniProt Synonym Gene Names: RBX2; ROC2; SAG; Rbx2; CKBBP1

NCBI Description

The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription (PubMed:10851089). CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (). Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.