Mouse Calpain 10 (CAPN10) ELISA Kit | CAPN10 elisa kit

Mouse Calpain 10 (CAPN10) ELISA Kit

Cat. Num. AAA2022333

• Gene Names: CAPN10; CANP10; NIDDM1
• Reactivity: Mouse
• Synonyms: Calpain 10 (CAPN10); Mouse Calpain 10 (CAPN10) ELISA Kit; Calcium-activated neutral proteinase 10; CAPN10 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Calpain 10 (CAPN10).; No significant cross-reactivity or interference between Calpain 10 (CAPN10) and analogues was observed.
• Sequence Length: 138

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.103ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calpain 10 (CAPN10) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calpain 10 (CAPN10) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAPN10 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain 10 (CAPN10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpain 10 (CAPN10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpain 10 (CAPN10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calpain 10 (CAPN10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CAPN10 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 37589919
• NCBI GeneID: 11132
• UniProt Accession #: Q9HC96; Q4ZFV1; Q8NCD4; Q96IG4; Q96JI2; Q9HC89; Q9HC90; Q9HC91; Q9HC92; Q9HC93; Q9HC94; A8MVS7
• Molecular Weight: 15,275 Da
• NCBI Official Full Name: Calpain 10
• NCBI Official Synonym Full Names: calpain 10
• NCBI Official Symbol: CAPN10
• NCBI Official Synonym Symbols: CANP10; NIDDM1
• NCBI Protein Information: calpain-10
• UniProt Protein Name: Calpain-10
• Protein Family: Calpain
• UniProt Gene Name: CAPN10
• UniProt Synonym Gene Names: KIAA1845; CANP 10
• UniProt Entry Name: CAN10_HUMAN

NCBI Description

Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. [provided by RefSeq, Sep 2010]

Uniprot Description

CAPN10: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Defects in CAPN10 are a cause of susceptibility to diabetes mellitus non-insulin-dependent type 1 (NIDDM1). It is a multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Belongs to the peptidase C2 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Apoptosis; EC 3.4.22.-; Mitochondrial

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: mitochondrion; cell; plasma membrane; cytosol

Molecular Function: SNARE binding; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity

Biological Process: positive regulation of glucose import; cellular response to insulin stimulus; positive regulation of intracellular transport; actin cytoskeleton reorganization; positive regulation of insulin secretion; proteolysis; cell structure disassembly during apoptosis

Disease: Diabetes Mellitus, Noninsulin-dependent, 1