Rabbit PTPN22 Polyclonal Antibody | anti-PTPN22 antibody

PTPN22 Polyclonal Antibody

Cat. Num. AAA3007626

• Gene Names: PTPN22; LYP; PEP; LYP1; LYP2; PTPN8; PTPN22.5; PTPN22.6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PTPN22; Polyclonal Antibody; PTPN22 Polyclonal Antibody; Tyrosine-protein phosphatase non-receptor type 22; Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; LyP; PEST-domain phosphatase; PEP; PTPN8; anti-PTPN22 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PTPN22 polyclonal antibody detects endogenous levels of PTPN22 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 779

• Applicable Applications for anti-PTPN22 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human PTPN22.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PTPN22 polyclonal antibody at 1:500 dilutionLane1:Jurkat whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:BV2 whole cell lysate (40ug))

Related Product Information for anti-PTPN22 antibody

The protein tyrosine phosphatase PTPN22 (PTP22, LYP, PEP, formerly PTPN8) is a genetic variant that confers risk of developing diverse human autoimmune diseases such as type 1 diabetes and rheumatoid arthritis. The minor allele of a missense SNP in PTPN22 encodes a hematopoietic-specific protein tyrosine phosphatase also known as "Lyp". The risk allele is present in about 17% of Caucasian individuals from the general population and in approximately 28% of Caucasian individuals with rheumatoid arthritis; it is thought to disrupt the P1 proline-rich motif that is important for interaction with the Src homology-3 (SH3) domain of CSK (cytoplasmic tyrosine kinase), potentially altering the normal functions of these proteins as negative regulators of T cell activation. The interaction between CSK and PTPN22 is highly specific and it is speculated that PTPN22 may be an effector and/or regulator of CSK in T cells and other hematopoietic cells.

NCBI and Uniprot Product Information

• NCBI GI #: 301171669
• NCBI GeneID: 26191
• NCBI Accession #: NP_001180360.1
• NCBI GenBank Nucleotide #: NP_001180360.1
• UniProt Accession #: Q9Y2R2
• Molecular Weight: ~ 98kDa
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 22 isoform 3
• NCBI Official Synonym Full Names: protein tyrosine phosphatase non-receptor type 22
• NCBI Official Symbol: PTPN22
• NCBI Official Synonym Symbols: LYP; PEP; LYP1; LYP2; PTPN8; PTPN22.5; PTPN22.6
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 22
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 22
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN22
• UniProt Synonym Gene Names: PTPN8; LyP; PEP
• UniProt Entry Name: PTN22_HUMAN

NCBI Description

This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Mar 2009]

Uniprot Description

PTPN22: Seems to act on Cbl. May play a role in regulating the function of Cbl and its associated protein kinases. Acts as negative regulator of T-cell receptor (TCR) signaling. Dephosphorylates and inactivates the SRC family kinases. Interacts with CSK. Interacts with LPXN. Predominantly expressed in lymphoid tissues and cells. Isoform 1 is expressed in thymocytes and both mature B and T-cells. Down-regulated by phosphorylation. Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: internal side of plasma membrane; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: protein binding; protein tyrosine phosphatase activity; kinase binding; SH3 domain binding

Biological Process: regulation of B cell receptor signaling pathway; regulation of natural killer cell proliferation; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation; negative regulation of T cell activation; T cell differentiation; regulation of innate immune response

Disease: Diabetes Mellitus, Insulin-dependent; Systemic Lupus Erythematosus; Rheumatoid Arthritis