SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
CTRP5/C1qTNF5 recombinant protein
Recombinant CTRP5/C1qTNF5
Applications
SDS-Page
Purity
>85% by SDS-PAGE
Synonyms
CTRP5/C1qTNF5; Recombinant CTRP5/C1qTNF5; Complement C1q tumor necrosis factor-related protein 5; CTRP5; MFRP; CTRP5/C1qTNF5 recombinant protein
Host
E Coli
Purity/Purification
>85% by SDS-PAGE
Form/Format
Liquid; 20mM Tris-HCl Buffer (pH8.5) Containing 30% Glycerol, 0.2M NaCl.
Concentration
0.25mg/ml (determined by BCA assay) (varies by lot)
Sequence Positions
16-243 aa
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSPPLD DNKIPSLCPG HPGLPGTPGH HGSQGLPGRD GRDGRDGAPG APGEKGEGGR PGLPGPRGDP GPRGEAGPAG PTGPAGECSV PPRSAFSAKR SESRVPPPSD APLPFDRVLV NEQGHYDAVT GKFTCQVPGV YYFAVHATVY RASLQFDLVK NGESIASFFQ FFGGWPKPAS LSGGAMVRLE PEDQVWVQVG VGDYIGIYAS IKTDSTFSGF LVYSDWHSSP VFA
Sequence Length
243
Applicable Applications for CTRP5/C1qTNF5 recombinant protein
SDS-PAGE
Species
Human
Endotoxin
<1.0 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Related Product Information for CTRP5/C1qTNF5 recombinant protein
CTRP5, also known as Complement C1q tumor necrosis factor-related protein 5, encodes a short-chain collagen which is strongly expressed in sub-retinal pigment epithelium (sub-RPE), ciliary epithelium and adipose tissue. CTRP5 is increased in mtDNA-depleted myocytes and that it stimulates the phosphorylation of AMP activated protein kinase. CTRP5 plays an important role in the adhesion of the retinal pigment epithelium (RPE) to the Bruch Membrane, and mutations are thought to impair the adhesion, resulting in sub-RPE deposits. Recombinant human CTRP5, fused to His-tag at N-terminals, was expressed in E Coli and purified by conventional chromatography techniques.
Product Categories/Family for CTRP5/C1qTNF5 recombinant protein
References
Park SY., et al. (2009) J Biol Chem. 284(41):27780-9. Hayward C., et al. (2003) Hum Mol Genet. 12(20):2657-67. Ayyagari R., et al. (2005) Invest Ophthalmol Vis Sci. 46(9):3363-71.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26.4 kDa (253 aa), confirmed by MALDI-TOF
NCBI Official Full Name
complement C1q tumor necrosis factor-related protein 5
UniProt Protein Name
Complement C1q tumor necrosis factor-related protein 5
UniProt Gene Name
C1QTNF5
UniProt Synonym Gene Names
CTRP5
UniProt Entry Name
C1QT5_HUMAN
Uniprot Description
C1QTNF5: Defects in C1QTNF5 are a cause of late-onset retinal degeneration (LORD). LORD is an autosomal dominant disorder characterized by onset in the fifth to sixth decade with night blindness and punctate yellow-white deposits in the retinal fundus, progressing to severe central and peripheral degeneration, with choroidal neovascularization and chorioretinal atrophy.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: collagen
Disease: Late-onset Retinal Degeneration
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Product Notes
The CTRP5/C1qTNF5 c1qtnf5 (Catalog #AAA201124) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 16-243 aa. AAA Biotech's CTRP5/C1qTNF5 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the CTRP5/C1qTNF5 c1qtnf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMSPPLD DNKIPSLCPG HPGLPGTPGH HGSQGLPGRD GRDGRDGAPG APGEKGEGGR PGLPGPRGDP GPRGEAGPAG PTGPAGECSV PPRSAFSAKR SESRVPPPSD APLPFDRVLV NEQGHYDAVT GKFTCQVPGV YYFAVHATVY RASLQFDLVK NGESIASFFQ FFGGWPKPAS LSGGAMVRLE PEDQVWVQVG VGDYIGIYAS IKTDSTFSGF LVYSDWHSSP VFA. It is sometimes possible for the material contained within the vial of "CTRP5/C1qTNF5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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