SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
NHLH1 recombinant protein
NHLH1, 1-133aa, Human, His tag, E Coli
Gene Names
NHLH1; HEN1; NSCL; NSCL1; bHLHa35
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
NHLH1; 1-133aa; Human; His tag; E Coli; Nescient helix loop helix 1; bHLHa35; HEN1; NSCL; NSCL1; NHLH1 recombinant protein
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT
Concentration
0.25 mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
1-133aa
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMMLNSDT MELDLPPTHS ETESGFSDCG GGAGPDGAGP GGPGGGQARG PEPGEPGRKD LQHLSREERR RRRRATAKYR TAHATRERIR VEAFNLAFAE LRKLLPTLPP DKKLSKIEIL RLAICYISYL NHVLDV
Applicable Applications for NHLH1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 2-8°C for one week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeating freeze and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for NHLH1 recombinant protein
The helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. NHLH1 may serve as DNA-binding protein and may be involved in the control of cell-type determination, possibly within the developing nervous system. Recombinant human NHLH1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for NHLH1 recombinant protein
References
Brown L., et al. (1992) Proc. Natl. Acad. Sci. u.S.A. 89:8492-8496; Lipkowitz S., et al. (1992) J. Biol. Chem. 267:21065-21071;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17.0kDa (156aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
NCBI Official Full Name
helix-loop-helix protein 1
NCBI Official Synonym Full Names
nescient helix-loop-helix 1
NCBI Official Symbol
NHLH1
NCBI Official Synonym Symbols
HEN1; NSCL; NSCL1; bHLHa35
NCBI Protein Information
helix-loop-helix protein 1
UniProt Protein Name
Helix-loop-helix protein 1
Protein Family
UniProt Gene Name
NHLH1
UniProt Synonym Gene Names
BHLHA35; HEN1; HEN-1; bHLHa35; NSCL-1
NCBI Description
The helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. Four members of this family have been clearly implicated in tumorigenesis via their involvement in chromosomal translocations in lymphoid tumors: MYC (MIM 190080), LYL1 (MIM 151440), E2A (MIM 147141), and SCL (MIM 187040).[supplied by OMIM, Nov 2002]
Uniprot Description
May serve as DNA-binding protein and may be involved in the control of cell-type determination, possibly within the developing nervous system.
Research Articles on NHLH1
Similar Products
Product Notes
The NHLH1 nhlh1 (Catalog #AAA205032) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-133aa with tag His-tag. AAA Biotech's NHLH1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the NHLH1 nhlh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMMLNSDT MELDLPPTHS ETESGFSDCG GGAGPDGAGP GGPGGGQARG PEPGEPGRKD LQHLSREERR RRRRATAKYR TAHATRERIR VEAFNLAFAE LRKLLPTLPP DKKLSKIEIL RLAICYISYL NHVLDV. It is sometimes possible for the material contained within the vial of "NHLH1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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