SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
HPCAL1 recombinant protein
HPCAL1, 1-193aa, Human, His tag, E Coli
Gene Names
HPCAL1; BDR1; HLP2; VILIP-3
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
HPCAL1; 1-193aa; Human; His tag; E Coli; Hippocalcin-like 1; BDR1; Calcium-binding protein BDR-1; Hippocalcin-like protein 1; HLP2; VILIP-3; visinin-like protein 3; VILIP3; HPCAL1 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
1-193aa
Sequence
MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVLQDLRENT EFTDHELQEW YKGFLKDCPT GHLTVDEFKK IYANFFPYGD ASKFAEHVFR TFDTNGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISRSE MLEIVQAIYK MVSSVMKMPE DESTPEKRTD KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA SQF
Sequence Length
193
Applicable Applications for HPCAL1 recombinant protein
SDS-PAGE
Tag Information
His-tag
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for HPCAL1 recombinant protein
HPCAL1, also known as VILIP-3, is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It is involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. Recombinant human HPCAL1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for HPCAL1 recombinant protein
References
Fu J., et al. (2009) J Biol Chem. 284 (40):27577-86.; Spilker C., et al. (2003) Mol Cell Neurosci. 24 (3):766-78.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24.4 kDa (213aa), confirmed by MALDI-TOF
NCBI Official Full Name
hippocalcin-like protein 1
NCBI Official Synonym Full Names
hippocalcin like 1
NCBI Official Symbol
HPCAL1
NCBI Official Synonym Symbols
BDR1; HLP2; VILIP-3
NCBI Protein Information
hippocalcin-like protein 1
UniProt Protein Name
Hippocalcin-like protein 1
Protein Family
UniProt Gene Name
HPCAL1
UniProt Synonym Gene Names
BDR1; VILIP-3
NCBI Description
The protein encoded by this gene is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. Several alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Apr 2012]
Uniprot Description
May be involved in the calcium-dependent regulation of rhodopsin phosphorylation.MiscellaneousProbably binds two or three calcium ions.
Research Articles on HPCAL1
Similar Products
Product Notes
The HPCAL1 hpcal1 (Catalog #AAA203498) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-193aa with tag His-tag. AAA Biotech's HPCAL1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the HPCAL1 hpcal1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVLQDLRENT EFTDHELQEW YKGFLKDCPT GHLTVDEFKK IYANFFPYGD ASKFAEHVFR TFDTNGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISRSE MLEIVQAIYK MVSSVMKMPE DESTPEKRTD KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA SQF. It is sometimes possible for the material contained within the vial of "HPCAL1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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