BPIFA1 recombinant protein

BPIFA1, 20-256aa, Human, His tag, E Coli

Cat. Num. AAA204992

• Gene Names: BPIFA1; LUNX; NASG; PLUNC; SPURT; SPLUNC1; bA49G10.5
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: BPIFA1; 20-256aa; Human; His tag; E Coli; BPI fold-containing family A member 1; BPI fold-containing family A; member 1; bA49G10.5; LPLuNC3; LuNX; NASG; PLuNC; SPuRT; BPIFA1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol
• Concentration: 0.5 mg/ml (determined by BCA assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSQFGGLPV PLDQTLPLNV NPALPLSPTG LAGSLTNALS NGLLSGGLLG ILENLPLLDI LKPGGGTSGG LLGGLLGKVT SVIPGLNNII DIKVTDPQLL ELGLVQSPDG HRLYVTIPLG IKLQVNTPLV GASLLRLAVK LDITAEILAV RDKQERIHLV LGDCTHSPGS LQISLLDGLG PLPIQGLLDS LTGILNKVLP ELVQGNVCPL VNEVLRGLDI TLVHDIVNML IHGLQFVIKV
• Sequence Length: 20-256aa

• Applicable Applications for BPIFA1 recombinant protein: SDS-PAGE
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for BPIFA1 recombinant protein

BPIFA1, also known as PLuNC, belongs to the short subfamily of PLuNC family proteins and have homology only to the Nterminal domains of BPI. It is a secreted protein that is expressed in the secretory ducts and submucosal glands of tracheobronchial tissues. BPIFA1 binds to lipopolysaccharide (LPS) in nasal lavage fluid (NLF) which points to its role in the inflammatory response of the upper airways after exposure to irritants. Decreased levels of BPIFA1 occur in the NLF of smokers and people who have been exposed to reactive epoxy chemicals, indicating that long-term exposure to airway irritants impairs the production of BPIFA1 in the upper respiratory tract. Recombinant human BPIFA1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for BPIFA1 recombinant protein

Immunology

References

Ghafouri B., et al. (2004) Biochem Biophys Acta. 1699:57-63.; Bingle C D., et al. (2002) Hum Mol Genet. 11: 937-943.

NCBI and Uniprot Product Information

• NCBI GI #: 7706119
• NCBI GeneID: 51297
• NCBI Accession #: NP_057667
• NCBI GenBank Nucleotide #: NM_016583.3
• UniProt Accession #: Q9NP55; Q9NZT0; A6XMV5; A8K9R3; E1P5M9
• Molecular Weight: 27.1 kDa (260aa), confirmed by MALDI-TOF
• NCBI Official Full Name: BPI fold-containing family A member 1
• NCBI Official Synonym Full Names: BPI fold containing family A member 1
• NCBI Official Symbol: BPIFA1
• NCBI Official Synonym Symbols: LUNX; NASG; PLUNC; SPURT; SPLUNC1; bA49G10.5
• NCBI Protein Information: BPI fold-containing family A member 1
• UniProt Protein Name: BPI fold-containing family A member 1
• Protein Family: BPI fold-containing family
• UniProt Gene Name: BPIFA1
• UniProt Synonym Gene Names: LUNX; NASG; PLUNC; SPLUNC1; SPURT; SPLUNC1

NCBI Description

This gene is the human homolog of murine plunc, and like the mouse gene, is specifically expressed in the upper airways and nasopharyngeal regions. The encoded antimicrobial protein displays antibacterial activity against Gram-negative bacteria. It is thought to be involved in inflammatory responses to irritants in the upper airways and may also serve as a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer. Multiple transcript variants resulting from alternative splicing in the 3' UTR have been detected, but the full-length nature of only three are known. [provided by RefSeq, Aug 2014]

Uniprot Description

Plays a role in the innate immune responses of the upper airways. Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae. Binds bacterial lipopolysaccharide (LPS). Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus. Plays a role in the airway inflammatory response after exposure to irritants. May attract macrophages and neutrophils. May be associated with tumor progression.