Typical Testing Data/Standard Curve
Rat Probetacellulin ELISA Kit | BTC elisa kit
Rat Probetacellulin ELISA Kit
Reactivity
Rat
Applications
ELISA
Synonyms
Probetacellulin; Rat Probetacellulin ELISA Kit; BTC elisa kit
Reactivity
Rat
Specificity
The Rat Probetacellulin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat Probetacellulin proteins within the range of 7.8 pg/ml - 500 pg/ml.
Sequence Length
177
Applicable Applications for BTC elisa kit
Sandwich ELISA (EIA)
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for BTC elisa kit
Principle of the Assay||The Rat Probetacellulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Probetacellulin in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat Probetacellulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Probetacellulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Probetacellulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Probetacellulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Background/Introduction: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
19,579 Da
NCBI Official Full Name
Betacellulin
UniProt Protein Name
Betacellulin
Protein Family
UniProt Gene Name
Btc
UniProt Entry Name
Q9JJM4_RAT
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Product Notes
The Rat BTC btc (Catalog #AAA8244684) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244684 ELISA Kit recognizes Rat BTC. AAA Biotech's Probetacellulin can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the BTC btc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Probetacellulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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