Human AGRP ELISA Kit | AGRP elisa kit

Human AGRP ELISA Kit

Cat. Num. AAA8248331

• Gene Names: AGRP; ART; AGRT; ASIP2
• Reactivity: Human
• Synonyms: AGRP; Human AGRP ELISA Kit; AGRT; ART; Agouti-related protein; AGRP elisa kit

• Reactivity: Human
• Specificity: The Human AGRP ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human AGRP proteins within the range of 7.8 pg/ml - 500 pg/ml.
• Sequence Length: 132

• Samples: Cell Culture Supernates, Serum, Plasma, Urine
• Sensitivity: <4 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGRP elisa kit

Principle of the Assay: The Human AGRP ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human AGRP in Cell Culture Supernates, Serum, Plasma, Urine. This assay employs an antibody specific for Human AGRP coated on a 96-well plate. Standards and samples are pipetted into the wells and AGRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human AGRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AGRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Agouti-related protein, also called Agouti-related peptide (AgRP), is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. In humans, the agouti-related peptide is encoded by the AGRP gene. AgRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators.

NCBI and Uniprot Product Information

• NCBI GI #: 4501995
• NCBI GeneID: 181
• NCBI Accession #: NP_001129.1
• NCBI GenBank Nucleotide #: NM_001138.1
• UniProt Accession #: O00253; O15459; Q2TBD9
• Molecular Weight: 14,440 Da
• NCBI Official Full Name: agouti-related protein
• NCBI Official Synonym Full Names: agouti related neuropeptide
• NCBI Official Symbol: AGRP
• NCBI Official Synonym Symbols: ART; AGRT; ASIP2
• NCBI Protein Information: agouti-related protein
• UniProt Protein Name: Agouti-related protein
• Protein Family: Agouti-related protein
• UniProt Gene Name: AGRP
• UniProt Synonym Gene Names: AGRT; ART
• UniProt Entry Name: AGRP_HUMAN

NCBI Description

This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009]

Uniprot Description

Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R (). Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner.