Rat CCL3 ELISA Kit | CCL3 elisa kit

Rat CCL3 ELISA Kit

Cat. Num. AAA824889

• Gene Names: Ccl3; Scya3; MIP-1a
• Reactivity: Rat
• Applications: ELISA
• Synonyms: CCL3; Rat CCL3 ELISA Kit; G0S19-1; MIP1A; SCYA3; C-C motif chemokine 3; G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein; CCL3 elisa kit

• Reactivity: Rat
• Specificity: The Rat CCL3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat CCL3 proteins within the range of 7.8 pg/ml - 500 pg/ml.
• Sequence Length: 92

• Applicable Applications for CCL3 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma
• Assay Type: Quantitative Sandwich
• Sensitivity: <1 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL3 elisa kit

Principle of the Assay: The MyBioSource' Rat CCL3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CCL3 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat CCL3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CCL3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.!!Background/Introduction: Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.

NCBI and Uniprot Product Information

• NCBI GI #: 40254794
• NCBI GeneID: 25542
• NCBI Accession #: NP_037157.2
• NCBI GenBank Nucleotide #: NM_013025.2
• UniProt Accession #: P50229; A1EC87
• Molecular Weight: 10,335 Da
• NCBI Official Full Name: C-C motif chemokine 3
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 3
• NCBI Official Symbol: Ccl3
• NCBI Official Synonym Symbols: Scya3; MIP-1a
• NCBI Protein Information: C-C motif chemokine 3; MIP-1-alpha; small inducible cytokine A3; small-inducible cytokine A3; macrophage inflammatory protein 1-alpha; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3)
• UniProt Protein Name: C-C motif chemokine 3
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl3
• UniProt Synonym Gene Names: Mip1a; Scya3; MIP-1-alpha
• UniProt Entry Name: CCL3_RAT

NCBI Description

mediates monocyte and neutrophil chemotaxis; may play a role in the pathogenesis of acute lung injury [RGD, Feb 2006]

Uniprot Description

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.