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Western Blot (WB) (BRDT N-term western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the BRDT antibody detected the BRDT protein (arrow).)

Rabbit anti-Human BRDT Polyclonal Antibody | anti-BRDT antibody

BRDT, NT (BRDT, Bromodomain testis-specific protein, Cancer/testis antigen 9, RING3-like protein)

Gene Names
BRDT; CT9; BRD6
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Synonyms
BRDT; Polyclonal Antibody; NT (BRDT; Bromodomain testis-specific protein; Cancer/testis antigen 9; RING3-like protein); Anti -BRDT; anti-BRDT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-BRDT antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western blot1:100-500
Immunogen
BRDT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8~38 amino acids from the N-terminal region of human BRDT.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BRDT N-term western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the BRDT antibody detected the BRDT protein (arrow).)

Western Blot (WB) (BRDT N-term western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the BRDT antibody detected the BRDT protein (arrow).)
Related Product Information for anti-BRDT antibody
BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.
Product Categories/Family for anti-BRDT antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
676
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
107,954 Da
NCBI Official Full Name
bromodomain testis-specific protein isoform a
NCBI Official Synonym Full Names
bromodomain, testis-specific
NCBI Official Symbol
BRDT
NCBI Official Synonym Symbols
CT9; BRD6
NCBI Protein Information
bromodomain testis-specific protein; RING3-like protein; cancer/testis antigen 9
UniProt Protein Name
Bromodomain testis-specific protein
UniProt Gene Name
BRDT
UniProt Synonym Gene Names
CT9
UniProt Entry Name
BRDT_HUMAN

NCBI Description

BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

BRDT: an atypical protein kinase of the BRD family. Possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. A testis-specific protein that is also expressed at a high level in non-small cell lung cancer

Protein type: Kinase, protein; Cancer Testis Antigen (CTA); Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; ATYPICAL group; BRD family

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: nucleus

Molecular Function: histone binding; transcription coactivator activity

Biological Process: chromatin remodeling; male meiosis I; transcription, DNA-dependent; regulation of RNA splicing; RNA splicing; spermatogenesis; cell differentiation; mRNA processing; male meiosis; positive regulation of transcription, meiotic

Research Articles on BRDT

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Product Notes

The BRDT brdt (Catalog #AAA6007895) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRDT, NT (BRDT, Bromodomain testis-specific protein, Cancer/testis antigen 9, RING3-like protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRDT can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western blot1:100-500. Researchers should empirically determine the suitability of the BRDT brdt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRDT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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