Chromatin Immunoprecipitation (ChIP)
(ChIP-Seq of BRD2 antibody (pAb). ChIP was performed using BRD2 polyclonal antibody with 30 ug chromatin from the DHL4 cell line and 6 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 16 million sequence tags were mapped to identify BRD2 binding sites. The image shows binding across a region of chromosome 1.)
Rabbit anti-Human BRD2 Polyclonal Antibody | anti-BRD2 antibody
BRD2 antibody (pAb)
Gene Names
BRD2; FSH; NAT; RNF3; FSRG1; RING3; D6S113E; O27.1.1; BRD2-IT1
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity Purified
Synonyms
BRD2; Polyclonal Antibody; BRD2 antibody (pAb); Bromodomain Containing 2; O27.1.1; RING3; anti-BRD2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-BRD2 antibody
Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq)
Application Notes
Applications Validated:
ChIP-Seq: 6ul per ChIP
ChIP-Seq: 6ul per ChIP
Immunogen
This BRD2 antibody was raised against a recombinant protein comprising amino acids 58-232 of human BRD2 (NP_005095).
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(ChIP-Seq of BRD2 antibody (pAb). ChIP was performed using BRD2 polyclonal antibody with 30 ug chromatin from the DHL4 cell line and 6 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 16 million sequence tags were mapped to identify BRD2 binding sites. The image shows binding across a region of chromosome 1.)
Chromatin Immunoprecipitation (ChIP)
(ChIP-Seq of BRD2 antibody (pAb). ChIP was performed using BRD2 polyclonal antibody with 30 ug chromatin from the DHL4 cell line and 6 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 16 million sequence tags were mapped to identify BRD2 binding sites. The image shows binding across a region of chromosome 1.)
Related Product Information for anti-BRD2 antibody
Short Description: Our BRD2 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation and ChIP-Seq; it has been shown to react with Human samples.
Background: BRD2 (Bromodomain-containing protein 2) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female Sterile Homeotic protein in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD2 causes elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. BRD2 has been shown to interact with E2F1 and with histone H4 acetylated at Lys12 via its two bromodomains. BRD2 may play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses.
Background: BRD2 (Bromodomain-containing protein 2) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female Sterile Homeotic protein in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD2 causes elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. BRD2 has been shown to interact with E2F1 and with histone H4 acetylated at Lys12 via its two bromodomains. BRD2 may play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses.
Product Categories/Family for anti-BRD2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52.8kDa (478aa)
NCBI Official Full Name
bromodomain-containing protein 2 isoform 1
NCBI Official Synonym Full Names
bromodomain containing 2
NCBI Official Symbol
BRD2
NCBI Official Synonym Symbols
FSH; NAT; RNF3; FSRG1; RING3; D6S113E; O27.1.1; BRD2-IT1
NCBI Protein Information
bromodomain-containing protein 2
UniProt Protein Name
Bromodomain-containing protein 2
Protein Family
UniProt Gene Name
BRD2
UniProt Synonym Gene Names
KIAA9001; RING3
UniProt Entry Name
BRD2_HUMAN
NCBI Description
This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene. [provided by RefSeq, Dec 2010]
Research Articles on BRD2
Similar Products
Product Notes
The BRD2 brd2 (Catalog #AAA389049) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRD2 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRD2 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq). Applications Validated: ChIP-Seq: 6ul per ChIP. Researchers should empirically determine the suitability of the BRD2 brd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
