IL1-alpha/IL1A recombinant protein

Human IL1-alpha/IL1A protein

Cat. Num. AAA9719011

• Gene Names: IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IL1-alpha/IL1A; Human IL1-alpha/IL1A protein; BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1; alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha; IL1-alpha/IL1A recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH7.5.
• Sequence: Ser113-Ala271

• Endotoxin: < 1 EU per ug of the protein as determined by the LAL method.
• Protein Construction: Amino acid sequence derived from Human IL-1alpha protein isoform (P01583-1, Ser113-Ala271) was expressed.
• Protein Length: The recombinant Human IL-1alpha protein consists of 160 amino acids (N-Met) and has a predicted molecular mass of 18kDa. The apparent molecular mass of the Human IL1alpha protein is approximately 18kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-1alpha protein using the buffer we provided not less than 100ug/mL, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized Human IL1alpha protein protein protein product should be stored desiccated below -18 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human IL1-alpha/IL1A protein)

Related Product Information for IL1-alpha/IL1A recombinant protein

Description: Recombinant Mouse IL1-alpha/IL1A protein expressed in E.coli.
Background: IL-1alpha a member of the interleukin 1 cytokine family. Interleukin 1 (IL-1) is a name that designates two proteins, IL-1alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. IL-1alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.

Product Categories/Family for IL1-alpha/IL1A recombinant protein

Cell Culture; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 27894330
• NCBI GeneID: 3552
• NCBI Accession #: NP_000566.3
• NCBI GenBank Nucleotide #: NM_000575.3
• UniProt Accession #: P01583; Q53QF9; Q7RU02
• Molecular Weight: 30,607 Da
• NCBI Official Full Name: interleukin-1 alpha proprotein
• NCBI Official Synonym Full Names: interleukin 1, alpha
• NCBI Official Symbol: IL1A
• NCBI Official Synonym Symbols: IL1; IL-1A; IL1F1; IL1-ALPHA
• NCBI Protein Information: interleukin-1 alpha; IL-1 alpha; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha
• UniProt Protein Name: Interleukin-1 alpha
• UniProt Gene Name: IL1A
• UniProt Synonym Gene Names: IL1F1; IL-1 alpha
• UniProt Entry Name: IL1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family.

Protein type: Motility/polarity/chemotaxis; Cytokine

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein binding; interleukin-1 receptor binding; copper ion binding; cytokine activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; apoptosis; positive regulation of interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; positive regulation of JNK cascade; fever; negative regulation of cell proliferation; cell proliferation; connective tissue replacement during inflammatory response; positive regulation of angiogenesis; response to copper ion; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; positive regulation of cytokine secretion