CST5 active protein

CST5, 26-142aa, Human, His tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA205929

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CST5; 26-142aa; Human; His tag; Baculovirus (Bioactivity Validated); Cystatin-D; CST5 active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: QSRTLAGGIH ATDLNDKSVQ RALDFAISEY NKVINKDEYY SRPLQVMAAY QQIVGGVNYY FNVKFGRTTC TKSQPNLDNC PFNDQPKLKE EEFCSFQINE VPWEDKISIL NYKCRKVHHH HHH
• Sequence Length: 142

• Applicable Applications for CST5 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: The IC50 value is < 2.0nM. The inhibitory function of Cystatin 5 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CST5 active protein

CST5, also known as Cystatin-D, is a novel member of the cystatin superfamily. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Two alleles of the cystatin D gene (CST5), encoding protein variants with either Cys or Arg as residue 26 in their 122-residue polypeptide chains, are present in the population. Recombinant human CST5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CST5 active protein

Proteases & Inhibitors

References

Balbin M. et al. (1994) J Biol Chem. 269:23156-23162.; Alvarez-Fernandez M. et al. (2005) J Biol Chem. 280:18221-18228.

NCBI and Uniprot Product Information

• NCBI GI #: 19882256
• NCBI GeneID: 1473
• NCBI Accession #: NP_001891
• NCBI GenBank Nucleotide #: NM_001900.4
• UniProt Accession #: P28325; Q5JRF5; Q9UCA0
• Molecular Weight: 14.36kDa (123aa) (13.5-18kDa in SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: cystatin-D
• NCBI Official Synonym Full Names: cystatin D
• NCBI Official Symbol: CST5
• NCBI Protein Information: cystatin-D
• UniProt Protein Name: Cystatin-D
• Protein Family: Cystatin
• UniProt Gene Name: CST5

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein found in saliva and tears. The encoded protein may play a protective role against proteinases present in the oral cavity. [provided by RefSeq, Jul 2008]

Uniprot Description

Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B.