CST7 recombinant protein

CST7, 20-1452aa, Human, His tag, Baculovirus

Cat. Num. AAA206031

• Gene Names: CST7; CMAP
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: CST7; 20-1452aa; Human; His tag; Baculovirus; Cystatin-F; Cystatin F; CMAP; CST7 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4).
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: GPSPDTCSQD LNSRVKPGFP KTIKTNDPGV LQAARYSVEK FNNCTNDMFL FKESRITRAL VQIVKGLKYM LEVEIGRTTC KKNQHLRLDD CDFQTNHTLK QTLSCYSEVW VVPWLQHFEV PVLRCHHHHH HH
• Sequence Length: 145

• Applicable Applications for CST7 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CST7 recombinant protein

CST7, also known as cystatin-F, contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. This gene encodes a glycosylated cysteine protease inhibitor with a putative role in immune regulation through inhibition of a unique target in the hematopoietic system. Expression of the protein has been observed in various human cancer cell lines established from malignant tumors. Recombinant human CST7, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CST7 recombinant protein

Proteases & Inhibitors

References

Schuttelkopf AW, et al (2006) J Biol Chem. 281 (24):16570-5.

NCBI and Uniprot Product Information

• NCBI GI #: 262263314
• NCBI GeneID: 8530
• NCBI Accession #: NP_003641
• NCBI GenBank Nucleotide #: NM_003650.3
• UniProt Accession #: O76096; Q6FH95; Q7Z4J8; Q9UED4
• Molecular Weight: 15.3kDa (132aa) (18-28kDa in SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: cystatin-F
• NCBI Official Synonym Full Names: cystatin F
• NCBI Official Symbol: CST7
• NCBI Official Synonym Symbols: CMAP
• NCBI Protein Information: cystatin-F
• UniProt Protein Name: Cystatin-F
• Protein Family: Cystatin
• UniProt Gene Name: CST7
• UniProt Synonym Gene Names: CMAP

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. This gene encodes a glycosylated cysteine protease inhibitor with a putative role in immune regulation through inhibition of a unique target in the hematopoietic system. Expression of the protein has been observed in various human cancer cell lines established from malignant tumors. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.