Cystatin D Recombinant Protein | CST5 recombinant protein

Cystatin D Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120322

• Purity: >95% as determined by SDS-PAGE
• Synonyms: Cystatin D; Cystatin D Protein; Human; Recombinant (His Tag); Human Cystatin D/CST5 Protein (His Tag); CST5 Protein; MGC71922 Protein; cystatin D; CST5 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val142

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the pro form of human Cystatin D (NP_001891.2)(Met 1-Val 142) with a C-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CST5 recombinant protein

Background: Cystatins are natural inhibitors of papain-like (family C1) and legumain-related (family C13) cysteine peptidases. The mammalian cystatin superfamily members are of three major types, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. As a member of type 2 cystatin, cystatin D is a single-domain protein and also has cysteine residues that form disulfide bridges. In contrast with the wider distribution of all the other family 2 cystatins, cystatin D is tissue-restricted expressed and has been found only in saliva and tears. and meanwhile, it displays an inhibition profile with a preferential inhibition on cathepsin S, H, L. Although the exact functions are largely unknown, it has reported that cystatin D is involved in the inhibition of virus replication and apoptosis.

References

Freije J.P., et al.,(1991), Structure and expression of the gene encoding cystatin D, a novel human cysteine proteinase inhibitor. J. Biol. Chem. 266:20538-20543.Freije J.P., et al., (1993), Human cystatin D. cDNA cloning, characterization of the Escherichia coli expressed inhibitor, and identification of the native protein in saliva.J. Biol. Chem. 268:15737-15744.Deloukas P., et al.,(2001), The DNA sequence and comparative analysis of human chromosome 20.Nature 414:865-871.

NCBI and Uniprot Product Information

• NCBI GI #: 19882256
• NCBI GeneID: 1473
• NCBI Accession #: NP_001891.2
• NCBI GenBank Nucleotide #: NM_001900.4
• UniProt Accession #: P28325; Q5JRF5; Q9UCA0
• Molecular Weight: 16,080 Da
• NCBI Official Full Name: cystatin-D
• NCBI Official Synonym Full Names: cystatin D
• NCBI Official Symbol: CST5
• NCBI Protein Information: cystatin-D
• UniProt Protein Name: Cystatin-D
• Protein Family: Cystatin
• UniProt Gene Name: CST5
• UniProt Entry Name: CYTD_HUMAN

Uniprot Description

CST5: Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B. Belongs to the cystatin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: extracellular region; extracellular space

Molecular Function: cysteine protease inhibitor activity; protease binding; protein binding