CST3 active protein

CST3, 27-146aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA205312

• Gene Names: CST3; ARMD11; HEL-S-2
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: CST3; 27-146aa; Human; His tag; E Coli (Bioactivity Validated); Cystatin C; ARMD11; MGC117328; Cystatin-C; CST3 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol.
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSSPGKPPRL VGGPMDASVE EEGVRRALDF AVGEYNKASN DMYHSRALQV VRARKQIVAG VNYFLDVELG RTTCTKTQPN LDNCPFHDQP HLKRKAFCSF QIYAVPWQGT MTLSKSTCQD A
• Sequence Length: 146

• Applicable Applications for CST3 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: The IC50 value is < 2.0nM. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CST3 active protein

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. CST3 is located in the cystatin locus and is the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. Recombinant human CST3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CST3 active protein

Proteases & Inhibitors

References

Liu J. (2012) Transplant Proc. 44 (5):1250-3.

NCBI and Uniprot Product Information

• NCBI GI #: 4503107
• NCBI GeneID: 1471
• NCBI Accession #: NP_000090
• NCBI GenBank Nucleotide #: NM_000099.3
• UniProt Accession #: P01034; Q6FGW9; B2R5J9; D3DW42
• Molecular Weight: 15.6 kDa (141aa) confirmed by MALDI-TOF
• NCBI Official Full Name: cystatin-C
• NCBI Official Synonym Full Names: cystatin C
• NCBI Official Symbol: CST3
• NCBI Official Synonym Symbols: ARMD11; HEL-S-2
• NCBI Protein Information: cystatin-C
• UniProt Protein Name: Cystatin-C
• Protein Family: Cystatin
• UniProt Gene Name: CST3

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014]

Uniprot Description

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.