SDS-Page
Cst3 active protein
Cst3, 21-140aa, Rat, His tag, Baculovirus (Bioactivity Validated)
Gene Names
Cst3; CYSC
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Cst3; 21-140aa; Rat; His tag; Baculovirus (Bioactivity Validated); Cystain-C; CST3; CYSC; Cst3 active protein
Host
Baculovirus
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
GTSRPPPRLL GAPQEADASE EGVQRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGI NYYLDVEMGR TTCTKSQTNL TNCPFHDQPH LMRKALCSFQ IYSVPWKGTH TLTKSSCKNA LEHHHHHH
Sequence Length
140
Applicable Applications for Cst3 active protein
SDS-PAGE
Antigen Species
Rat
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
The IC50 value is < 1.0nM. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for Cst3 active protein
CST3, also known as cystain-C, is secreted type 2 cysteine proteinase inhibitors of the cystatin superfamily. It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S. Recombinant rat CST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for Cst3 active protein
References
Reed CH., et al. (2000) Br J Biomed. Sci. 57:323-329.; Esnard A., et al. (1992) FEBS Lett. 300:131-135.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14.3kDa (128aa) 13.5-18KDa (SDS-PAGE under reducing conditions.)
NCBI Official Full Name
cystatin-C
NCBI Official Synonym Full Names
cystatin C
NCBI Official Symbol
Cst3
NCBI Official Synonym Symbols
CYSC
NCBI Protein Information
cystatin-C
UniProt Protein Name
Cystatin-C
Protein Family
UniProt Gene Name
Cst3
NCBI Description
extracellular cysteine protease inhibitor that may be involved in amyloidosis [RGD, Feb 2006]
Uniprot Description
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.
Research Articles on Cst3
Similar Products
Product Notes
The Cst3 cst3 (Catalog #AAA206152) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's Cst3 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Cst3 cst3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GTSRPPPRLL GAPQEADASE EGVQRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGI NYYLDVEMGR TTCTKSQTNL TNCPFHDQPH LMRKALCSFQ IYSVPWKGTH TLTKSSCKNA LEHHHHHH. It is sometimes possible for the material contained within the vial of "Cst3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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