Rabbit anti-Rat Beta-2 microglobulin Monoclonal Antibody | anti-B2M antibody

Recombinant Anti-Beta-2 microglobulin Antibody, Rabbit Monoclonal

Cat. Num. AAA8113004

• Reactivity: Rat
• Applications: ELISA, ELISA, Immunohistochemistry
• Purity: Protein A
• Synonyms: Beta-2 microglobulin; Monoclonal Antibody; Recombinant Anti-Beta-2 microglobulin Antibody; Rabbit Monoclonal; B2M/beta-2 microglobulin Antibody; Rabbit MAb; beta-2 microglobulin; anti-B2M antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 035
• Specificity: Rat B2M/beta-2 microglobulin
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-B2M antibody: ELISA (EIA), ELISA (EIA) (Det), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:100-1:500; ELISA (Det): 1:1000-1:10,000; ELISA (Det): This antibody will detect Rat Beta-2 microglobulin/B2M in ELISA pair set.; In a sandwich ELISA, it can be used as detection antibody when paired with.
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Rat B2M/beta-2 microglobulin Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Rat B2M/beta-2 microglobulin (rR B2M/beta-2 microglobulin).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of rat B2M in rat thymus with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of rat B2M in rat spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-B2M antibody

B2M, also known as beta2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (beta2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (beta2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens. B2M (beta2-Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the alpha3 chain. Unlike alpha3, beta2 has no transmembrane region. Directly above beta2 lies the alpha1 chain, which itself is lateral to the alpha2. In the absence of B2M (beta2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (beta2 microglobulin) can indicate non-progression of HIV.

References

Poulik MD, et al. (1979) Beta 2-Microglobulin: methods and clinical applications. CRC Ctit Rev Clin Lab Sci. 10(3): 225-45.Poulik MD, et al. (1975) Beta2-Microglobulins. Contemp Top Mol Immunol. 4: 157-204.Berggard I. (1976) Beta2-Microglobulins: isolation, properties, and distribution. Fed Proc. 35(5): 1167-70.

NCBI and Uniprot Product Information

• NCBI GI #: 7549746
• NCBI GeneID: 24223
• NCBI Accession #: NP_036644.1
• NCBI GenBank Nucleotide #: NM_012512.2
• UniProt Accession #: P07151
• Molecular Weight: 13,720 Da
• NCBI Official Full Name: beta-2-microglobulin
• NCBI Official Synonym Full Names: beta-2 microglobulin
• NCBI Official Symbol: B2m
• NCBI Protein Information: beta-2-microglobulin; beta-2-microglobulin C-terminal fragment (55 AA)
• UniProt Protein Name: Beta-2-microglobulin
• Protein Family: Beta-2-microglobulin
• UniProt Gene Name: B2m
• UniProt Entry Name: B2MG_RAT

NCBI Description

mouse homolog plays a role in the assembly and surface expression of MHC class I molecules [RGD, Feb 2006]

Uniprot Description

B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: Golgi apparatus; extracellular space; focal adhesion; membrane; cytoplasm; plasma membrane; MHC class I protein complex; external side of plasma membrane

Molecular Function: identical protein binding

Biological Process: response to drug; retinal homeostasis; antigen processing and presentation of peptide antigen via MHC class I; iron ion homeostasis; response to cadmium ion; positive regulation of T cell mediated cytotoxicity; response to molecule of bacterial origin; T cell differentiation in the thymus; protein refolding; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent