Western Blot (WB)
((1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SERPINB1 Polyclonal Antibody | anti-SERPINB1 antibody
Goat anti-SERPINB1 Antibody
Gene Names
SERPINB1; EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2; HEL-S-27
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
SERPINB1; Polyclonal Antibody; Goat anti-SERPINB1 Antibody; Goat Anti-SERPINB1 (aa130-143) Antibody; serpin peptidase inhibitor; clade B (ovalbumin); member 1; EI; ELANH2; LEI; M/NEI; MNEI; PI-2; PI2; leukocyte elastase inhibitor; peptidase inhibitor 2; protease inhibitor 2 (anti-elastase); monocyte/neutrophil derived; serine (or cysteine) prot; SERPINB1 antibody; member 1 antibody; EI antibody; ELANH2 antibody; LEI antibody; M/NEI antibody; MNEI antibody; PI-2 antibody; PI2 antibody; leukocyte elastase inhibitor antibody; peptidase inhibitor 2 antibody; monocyte/neutrophil derived antibody; serine (or cysteine) prot antibody; anti-SERPINB1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EVHSRFQSLNADINKR
Sequence Length
379
Applicable Applications for anti-SERPINB1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 43kDa band observed in lysates of cell line A549 (calculated MW of 42.7kDa according to NP_109591.1). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 43kDa band observed in lysates of cell line A549 (calculated MW of 42.7kDa according to NP_109591.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-EVHSRFQSLNADINKR, from the internal region of the protein sequence according to NP_109591.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,073 Da
NCBI Official Full Name
leukocyte elastase inhibitor
NCBI Official Synonym Full Names
serpin family B member 1
NCBI Official Symbol
SERPINB1
NCBI Official Synonym Symbols
EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2; HEL-S-27
NCBI Protein Information
leukocyte elastase inhibitor
UniProt Protein Name
Leukocyte elastase inhibitor
Protein Family
UniProt Gene Name
SERPINB1
UniProt Synonym Gene Names
ELANH2; MNEI; PI2; LEI; EI; M/NEI; PI-2
UniProt Entry Name
ILEU_HUMAN
NCBI Description
The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Uniprot Description
SERPINB1: Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Belongs to the serpin family. Ov-serpin subfamily.
Chromosomal Location of Human Ortholog: 6p25
Cellular Component: cytoplasm; extracellular space; membrane
Molecular Function: serine-type endopeptidase inhibitor activity
Research Articles on SERPINB1
Similar Products
Product Notes
The SERPINB1 serpinb1 (Catalog #AAA423467) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SERPINB1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's SERPINB1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 43kDa band observed in lysates of cell line A549 (calculated MW of 42.7kDa according to NP_109591.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the SERPINB1 serpinb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EVHSRFQSLN ADINKR. It is sometimes possible for the material contained within the vial of "SERPINB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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