SDS-PAGE
(SDS-PAGE analysis of recombinant ATG12 on Coomassie Blue-stained 4 - 12% SDS-PAGE gel.)
ATG12 recombinant protein
ATG12 Recombinant Protein
Gene Names
Atg12; Apg12l; Atg12l; 4931423H11Rik; A330058M13Rik
Applications
ELISA, Western Blot
Purity
~95%
Synonyms
ATG12; ATG12 Recombinant Protein; APG12; FBR93; APG12L; HAPG12; Ubiquitin-like protein ATG12; Autophagy-related protein 12; APG12-like; ATG12 autophagy related 12 homolog (S. cerevisiae); ATG12 recombinant protein
Purity/Purification
~95%
Form/Format
Liquid
Sequence
aa 101 - 141
Sequence Length
141
Applicable Applications for ATG12 recombinant protein
ELISA (EIA), Western Blot (WB), MS
Application Notes
This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
Species
Mouse
Source
E Coli
Buffer
1X PBS containing 0.1% SDS
Fusion Tag
Fusion Partner: C-terminal His-tag
Preparation and Storage
Store in working aliquots at -70 degree C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
SDS-PAGE
(SDS-PAGE analysis of recombinant ATG12 on Coomassie Blue-stained 4 - 12% SDS-PAGE gel.)
SDS-PAGE
(SDS-PAGE analysis of recombinant ATG12 on Coomassie Blue-stained 4 - 12% SDS-PAGE gel.)
Related Product Information for ATG12 recombinant protein
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy (4). This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells (5). ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7 (6).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
10 kDa (Calculated)
NCBI Official Full Name
Ubiquitin-like protein ATG12
NCBI Official Synonym Full Names
autophagy related 12
NCBI Official Symbol
Atg12
NCBI Official Synonym Symbols
Apg12l; Atg12l; 4931423H11Rik; A330058M13Rik
NCBI Protein Information
ubiquitin-like protein ATG12; autophagy-related 12; autophagy-related protein 12
UniProt Protein Name
Ubiquitin-like protein ATG12
Protein Family
UniProt Gene Name
Atg12
UniProt Synonym Gene Names
Apg12; Apg12l; APG12-like
UniProt Entry Name
ATG12_MOUSE
Uniprot Description
ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Autophagy; Ubiquitin-like modifier
Chromosomal Location of Human Ortholog: 5q21-q22
Cellular Component: pre-autophagosomal structure membrane
Molecular Function: protein binding; APG8 conjugating enzyme activity
Biological Process: mitochondrion degradation; innate immune response; C-terminal protein lipidation; negative regulation of interferon type I production; autophagic vacuole formation; cellular response to nitrogen starvation
Research Articles on ATG12
Similar Products
Product Notes
The ATG12 atg12 (Catalog #AAA153294) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ATG12 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), MS. This recombinant protein can be used for WB, ELISA, MS and neutralization assays. Researchers should empirically determine the suitability of the ATG12 atg12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: aa 101 - 141. It is sometimes possible for the material contained within the vial of "ATG12, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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