Rabbit anti-Mouse, Rat ATG12 Polyclonal Antibody | anti-ATG12 antibody

RABBIT ANTI ATG12 (N-TERMINAL)

Cat. Num. AAA223848

• Gene Names: Atg12; Apg12l
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: ATG12; Polyclonal Antibody; RABBIT ANTI ATG12 (N-TERMINAL); anti-ATG12 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 141

• Applicable Applications for anti-ATG12 antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 40 kDa in human brain tissue lysate.; Immunohistology - Paraffin: Minimum Dilution: 2.5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide corresponding to a 16 aa sequence from near the amino terminus of human ATG12. ; Histology Positive Control Tissue
• Antiserum Preparation: Antiserum to human ATG12 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of human brain lysate probed with Rabbit anti Human ATG12 (MBS223848) at 0.5(A) and 1(B) ug/ml)

Testing Data #2

(Immunohistochemical staining of human brain with Rabbit anti Human ATG12 (MBS223848))

Related Product Information for anti-ATG12 antibody

Rabbit anti ATG12 (N-Terminal) antibody recognizes human Autophagy-related protein 12 (ATG12). ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA, inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. There are at least two isoforms of ATG12.

NCBI and Uniprot Product Information

• NCBI GI #: 84042517
• NCBI GeneID: 361321
• NCBI Accession #: NP_001033584.1
• NCBI GenBank Nucleotide #: NM_001038495.1
• Molecular Weight: 15,284 Da
• NCBI Official Full Name: ubiquitin-like protein ATG12
• NCBI Official Synonym Full Names: autophagy related 12
• NCBI Official Symbol: Atg12
• NCBI Official Synonym Symbols: Apg12l
• NCBI Protein Information: ubiquitin-like protein ATG12; ATG12 autophagy related 12 homolog; autophagy-related 12; autophagy-related protein 12
• UniProt Protein Name: Ubiquitin-like protein ATG12
• Protein Family: Autophagy protein
• UniProt Gene Name: Atg12
• UniProt Synonym Gene Names: Apg12l; APG12-like
• UniProt Entry Name: ATG12_RAT

Uniprot Description

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus ().