Rabbit Apg12, IN Polyclonal Antibody | anti-ATG12 antibody

Apg12, IN (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)

Cat. Num. AAA620694

• Gene Names: ATG12; APG12; FBR93; APG12L; HAPG12
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Apg12; IN; Polyclonal Antibody; IN (Autophagy-related 12; 4931423H11Rik; A330058M13Rik; Atg12; Apg12L; APG12-like; Atg12L; Autophagy-related protein 12); Anti -Apg12; anti-ATG12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATG12. Species crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-ATG12 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-1ug/ml
• Immunogen: Synthetic peptide corresponding to 15aa from near the center of human ATG12 (Genbank accession No. EAW48955).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of ATG12 in mouse heart tissue lysate with ATG12 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)

Related Product Information for anti-ATG12 antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy (4). This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells (5). ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7 (6). At least two isoforms of ATG12 are known to exist.

Product Categories/Family for anti-ATG12 antibody

Antibodies; Abs to Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 290560746
• NCBI GeneID: 9140
• NCBI Accession #: NP_004698.3
• NCBI GenBank Nucleotide #: NM_004707.3
• UniProt Accession #: O94817; Q6PJV2
• Molecular Weight: 15,113 Da
• NCBI Official Full Name: ubiquitin-like protein ATG12
• NCBI Official Synonym Full Names: ATG12 autophagy related 12 homolog (S. cerevisiae)
• NCBI Official Symbol: ATG12
• NCBI Official Synonym Symbols: APG12; FBR93; APG12L; HAPG12
• NCBI Protein Information: ubiquitin-like protein ATG12; OTTHUMP00000159129; autophagy-related protein 12; Apg12 (autophagy, yeast) homolog
• UniProt Protein Name: Ubiquitin-like protein ATG12
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG12
• UniProt Synonym Gene Names: APG12; APG12L
• UniProt Entry Name: ATG12_HUMAN

NCBI Description

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]

Uniprot Description

ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: pre-autophagosomal structure membrane

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: mitochondrion degradation; innate immune response; C-terminal protein lipidation; negative regulation of interferon type I production; cellular response to nitrogen starvation; autophagic vacuole formation