Mouse anti-Human Apg12 Monoclonal Antibody | anti-ATG12 antibody

Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)

Cat. Num. AAA608100

• Gene Names: ATG12; APG12; FBR93; APG12L; HAPG12
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Apg12; Monoclonal Antibody; Apg12 (Autophagy-related 12; 4931423H11Rik; A330058M13Rik; Atg12; Apg12L; APG12-like; Atg12L; Autophagy-related protein 12); Anti -Apg12 (Autophagy-related 12; anti-ATG12 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9G313
• Specificity: Recognizes human Atg12.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.

• Applicable Applications for anti-ATG12 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.
• Immunogen: Full recombinant human APG12, isoform 2, (1-74) with a GST tag.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degree C. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot detection of GST tagged recombinant human APG12. Note the GST tag alone is 26 kD.)

ELISA (EIA)

(Detection limit of 1 ng/ml for GST tagged recombinant APG12 when A2298-76J, is used as a capture antibody in sandwich ELISA.)

Related Product Information for anti-ATG12 antibody

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. APG12 is the human homolog of a yeast protein involved in autophagy. APG12 is conjugated to APG5. The APG12-ATG5 conjugate has an apparent molecular mass of 65kD. The C-terminal glycine of APG12 is conjugated to a central lysine (lys130) of APG5. Studies showed that overexpression of APG3 facilitated formation of the APG12-APG5 conjugate, suggesting that APG3 cross-talks with the APG12 conjugation system. APG12 is ubiquitously expressed.

Product Categories/Family for anti-ATG12 antibody

Antibodies; Abs to Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 290560746
• NCBI GeneID: 9140
• NCBI Accession #: NP_004698.3
• NCBI GenBank Nucleotide #: NM_004707.3
• UniProt Accession #: O94817; Q6PJV2
• Molecular Weight: 15,113 Da
• NCBI Official Full Name: ubiquitin-like protein ATG12
• NCBI Official Synonym Full Names: ATG12 autophagy related 12 homolog (S. cerevisiae)
• NCBI Official Symbol: ATG12
• NCBI Official Synonym Symbols: APG12; FBR93; APG12L; HAPG12
• NCBI Protein Information: ubiquitin-like protein ATG12; OTTHUMP00000159129; autophagy-related protein 12; Apg12 (autophagy, yeast) homolog
• UniProt Protein Name: Ubiquitin-like protein ATG12
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG12
• UniProt Synonym Gene Names: APG12; APG12L
• UniProt Entry Name: ATG12_HUMAN

NCBI Description

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]

Uniprot Description

ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-like modifier; Autophagy

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: pre-autophagosomal structure membrane

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: mitochondrion degradation; innate immune response; C-terminal protein lipidation; negative regulation of interferon type I production; cellular response to nitrogen starvation; autophagic vacuole formation