Western Blot (WB)
(Western blot analysis of APG12 in 3T3 cell lysate (Lane 1) and rat kidney tissue lsyate (Lane 2).)
Rabbit ATG12 Polyclonal Antibody | anti-ATG12 antibody
ATG12 Antibody
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
ATG12; Polyclonal Antibody; ATG12 Antibody; Rabbit Polyclonal ATG12 Antibody; Polyclonal ATG12 antibody; anti-ATG12 antibody; HAPG12 antibody; ATG 12 antibody; ATG-12; APG12 antibody; ATG 12; APG12L antibody; ATG-12 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer containing 30% Glycerol, 0.5 mg/ml BSA and 0.01% Thimerosal
Concentration
500 ug/ml (varies by lot)
Sequence Length
187
Applicable Applications for anti-ATG12 antibody
Western Blot (WB)
Application Notes
WB: 0.5-4 ug/ml
Immunogen
ATG12 antibody was raised in Rabbit using synthetic peptide surrounding amino acid 21 of mouse APG12 as the immunogen.
Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of APG12 in 3T3 cell lysate (Lane 1) and rat kidney tissue lsyate (Lane 2).)
Western Blot (WB)
(Western blot analysis of APG12 in 3T3 cell lysate (Lane 1) and rat kidney tissue lsyate (Lane 2).)
Related Product Information for anti-ATG12 antibody
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase -like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA, inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.
Product Categories/Family for anti-ATG12 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Atg12
NCBI Official Synonym Full Names
autophagy related 12
NCBI Official Symbol
ATG12
NCBI Official Synonym Symbols
APG12; FBR93; APG12L; HAPG12
NCBI Protein Information
ubiquitin-like protein ATG12
Protein Family
NCBI Description
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
Research Articles on ATG12
Similar Products
Product Notes
The ATG12 (Catalog #AAA5311539) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ATG12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.5-4 ug/ml. Researchers should empirically determine the suitability of the ATG12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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