ATG5 recombinant protein

ATG5 Recombinant Protein

Cat. Num. AAA153315

• Gene Names: ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
• Applications: ELISA, Western Blot
• Purity: ~95%
• Synonyms: ATG5; ATG5 Recombinant Protein; ASP; APG5; APG5L; hAPG5; APG5-LIKE; ATG5 autophagy related 5 homolog (S. cerevisiae); ATG5 recombinant protein

• Purity/Purification: ~95%
• Form/Format: Liquid
• Sequence: aa 1 - 275
• Sequence Length: 275

• Applicable Applications for ATG5 recombinant protein: ELISA (EIA), Western Blot (WB), MS
• Application Notes: This recombinant protein can be used for WB, ELISA, MS and neutralization assays. For research use only.
• Species: Mouse
• Source: E Coli
• Buffer: 1X PBS containing 10% glycerol
• Fusion Tag: Fusion Partner: C-terminal His-tag
• Preparation and Storage: Store in working aliquots at -70 degree C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

SDS-PAGE

(SDS-PAGE analysis of recombinant ATG5 on Coomassie Blue-stained 4 - 12% SDS-PAGE gel.)

Related Product Information for ATG5 recombinant protein

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy (4). This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells (5).

NCBI and Uniprot Product Information

• NCBI GI #: 10285391
• NCBI GeneID: 9474
• UniProt Accession #: A9UGY9; Q9H1Y0
• Molecular Weight: 34 kDa (Calculated)
• NCBI Official Full Name: AV683528 GKC Homo sapiens cDNA clone GKCDAE12 5-, mRNA sequence
• NCBI Official Synonym Full Names: autophagy related 5
• NCBI Official Symbol: ATG5
• NCBI Official Synonym Symbols: ASP; APG5; APG5L; hAPG5; APG5-LIKE
• NCBI Protein Information: autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog
• UniProt Protein Name: Autophagy protein 5
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG5
• UniProt Entry Name: A9UGY9_HUMAN

Uniprot Description

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Ubiquitin conjugating system; Autophagy

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: membrane; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; axoneme

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; autophagy; innate immune response; blood vessel remodeling; negative regulation of protein ubiquitination; negative regulation of interferon type I production; otolith development; negative regulation of apoptosis; autophagic vacuole formation