Principle of the Assay: Under the action of reductant, iron ions in samples can be reduced into ferrous ions (FE2+). The latter then bind to probe and form complexes, which has a maximum absorption peak at 593nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly.
Background/Introduction: Iron is one of the metal elements in organism and has important physiological functions. Ferrous ion is a key element in heme and hemoglobin and plays an important role in many biochemical reactions. In recent years, with the introduction of the concept of iron death, it has been found that the absorption, transportation, storage and utilization of iron ions and their excessive accumulation in cells have a significant relationship with aging and disease.
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Product Notes
The Total Iron (Catalog #AAA2567950) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Total Iron, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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