Typical Testing Data/Standard Curve (for reference only)
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Toal Glutathione (T-GSH) Assay Kit | T-GSH assay kit
Toal Glutathione (T-GSH) Colorimetric Assay Kit
Synonyms
Toal Glutathione (T-GSH); Toal Glutathione (T-GSH) Colorimetric Assay Kit; Toal Glutathione; T-GSH; t-GSH; T-GSH assay kit
Application Notes
GSSG is reduced to GSH using Glutathione Reductase (GR). GSH reacts with 5,5'-dithiobis-bis-(2-nitrobenoicacid) to form 2-nitrobenoicacid which has a characteristic absorption peak at 412nm, the content of total glutathione is determined then. It can test samples of tissues, bacteria, fungus, cells, serums, ect.
Samples
Animal and plant tissues, bacteria, cells, serum
Detection Range
2-200 ug/mL
Sensitivity
2 ug/mL
Preparation and Storage
Store at -20 degree C and keep from light immediately upon receipt. Kit has a storage time of est. 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping: Gel pack with blue ice.
Shipping: Gel pack with blue ice.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for T-GSH assay kit
Principle of the Assay: Glutathione is a Tripeptide of Glycine, Glutamic acid and Cysteine. In the red blood cell, reduced Glutathione (GSH) is the key to maintain hemoglobin in reduced state and protect cells from Oxidative damage. GSH is the most important antioxidant sulfhydryl compound in cells, which plays an important role in oxidation resistance, protein sulfhydryl protection and amino acid transport across membrane. The ratio between reduced and oxidized Glutathione (GSH/GSSG) is the main indicator of the cell's redox state. Therefore, measuring the content of GSH and GSSG and the ratio of GSH/GSSG in cells can reflect the redox state of cells. CheKine™ Total Glutathione (T-GSH) Colorimetric Assay Kit can detect samples of Animal and Plant Tissues, Bacteria, Cells, Serum, etc. Glutathione reductase (glutathione reductase, GR) is used to reduce GSSG to GSH. GSH can react with 5,5'-Dithiobis-(2-Nitrobenzoic Acid) (5,5'-Dithiobis-2-Nitrobenzoic Acid, DTNB) to form 2-Nitro-5-Diol Benzoic Acid. This substance is yellow and has a maximum light absorption at a wavelength of 412 nm. In this way, the total glutathione content in the sample can be determined.
Product Categories/Family for T-GSH assay kit
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Product Notes
The T-GSH (Catalog #AAA9719027) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. GSSG is reduced to GSH using Glutathione Reductase (GR). GSH reacts with 5,5'-dithiobis-bis-(2-nitrobenoicacid) to form 2-nitrobenoicacid which has a characteristic absorption peak at 412nm, the content of total glutathione is determined then. It can test samples of tissues, bacteria, fungus, cells, serums, ect. Researchers should empirically determine the suitability of the T-GSH for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Toal Glutathione (T-GSH), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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