Rabbit anti-Human Wnt5a Polyclonal Antibody | anti-Wnt5a antibody

Wnt5a Antibody

Cat. Num. AAA8000697

• Gene Names: WNT5A; hWNT5A
• Reactivity: Human
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: Wnt5a; Polyclonal Antibody; Wnt5a Antibody; Rabbit Anti-Human Wnt5a Polyclonal; WNT5A_HUMAN Antibody; Protein Wnt 5a Antibody; WNT 5A protein Antibody; Wingless type MMTV integration site family member 5A Antibody; Wnt 5a Antibody; Protein Wnt-5a Antibody; WNT5A Antibody; hWNT 5A Antibody; hWNT5A Antibody; WNT 5A protein precursor Antibody; WNT5A protein precursor Antibody; Protein Wnt5a Antibody; anti-Wnt5a antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Detects 42.3 kDa, 35 kDa. 35 kDa band is another isoform.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 365

• Applicable Applications for anti-Wnt5a antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Target: Wnt5a
• Immunogen: Synthetic peptide of Human Wnt5a (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Immunogen Species: Human
• Cellular Localization: Secreted; Extracellular Space; Extracellular Matrix
• Conjugate: Unconjugated
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Tissue Specificity: Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level).
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~42.3 kDa Wnt5a protein using Rabbit Anti-Wnt5a Polyclonal Antibody (SPC-737). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt5a Polyclonal Antibody (SPC-737) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~42.3 kDa. Other Band(s): ~35 kDa isoform 2.)

Product Categories/Family for anti-Wnt5a antibody

Antibodies; Stem Cells; Signaling Pathways; Wnt/Beta Catenin Pathway; Cancer; Tumor suppressors; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 371502087
• NCBI GeneID: 7474
• NCBI Accession #: NP_001243034.1
• NCBI GenBank Nucleotide #: NM_001256105.1
• UniProt Accession #: P41221; Q6P278; A8K4A4
• Molecular Weight: 40,887 Da
• NCBI Official Full Name: protein Wnt-5a isoform 2
• NCBI Official Synonym Full Names: Wnt family member 5A
• NCBI Official Symbol: WNT5A
• NCBI Official Synonym Symbols: hWNT5A
• NCBI Protein Information: protein Wnt-5a
• UniProt Protein Name: Protein Wnt-5a
• Protein Family: Protein
• UniProt Gene Name: WNT5A

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed:15735754). Mediates motility of melanoma cells (PubMed:17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes ().