Typical Testing Data/Standard Curve (for reference only)
HDAC8 assay kit
HDAC8 Inhibitor Screening Kit (Fluorometric)
Synonyms
HDAC8; HDAC8 Inhibitor Screening Kit (Fluorometric); HDAC8 assay kit
Samples
Testing candidate HDAC8 inhibitors
Features & Benefits
Simple procedure; takes ~ 60 min
Fast and convenient
Kit contains the necessary reagents for testing putative HDAC-8 inhibitors
Fast and convenient
Kit contains the necessary reagents for testing putative HDAC-8 inhibitors
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HDAC8 assay kit
Product Information: HDAC8 Inhibitor Screening Assay Kit: High-Throughput Suitable Fluorometric Assay to Screen HDAC8 Inhibitors. Rapid, Reliable, Simple & Sensitive. Includes Inhibitor Control, Trichostatin A. 100 Assays.
Background: Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In MyBioSource's HDAC-8 Inhibitor screening Kit, HDAC-8 deacetylates the substrate (R-H-K(Ac)-K(Ac)-AFC) followed by an enzymatic cleavage to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. In the presence of an HDAC-8 inhibitor, the deacetylation will be impeded which prevents the cleavage of the substrate to release AFC. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC-8 inhibitors. Trichostatin A is included as a control inhibitor to compare the efficacy of the test inhibitors.
Summary: Detection method- Fluorescence (Em/Ex = 380/500 nm)
Applications- Suitable for high throughput screening of HDAC-8 inhibitors
Background: Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In MyBioSource's HDAC-8 Inhibitor screening Kit, HDAC-8 deacetylates the substrate (R-H-K(Ac)-K(Ac)-AFC) followed by an enzymatic cleavage to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. In the presence of an HDAC-8 inhibitor, the deacetylation will be impeded which prevents the cleavage of the substrate to release AFC. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC-8 inhibitors. Trichostatin A is included as a control inhibitor to compare the efficacy of the test inhibitors.
Summary: Detection method- Fluorescence (Em/Ex = 380/500 nm)
Applications- Suitable for high throughput screening of HDAC-8 inhibitors
Product Categories/Family for HDAC8 assay kit
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Product Notes
The HDAC8 (Catalog #AAA841575) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HDAC8, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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