Chicken Total Spleen tyosine kinase ELISA Kit | Syk elisa kit

Chicken Total Spleen tyosine kinase ELISA Kit

Cat. Num. AAA744549

• Gene Names: Syk; Sykb
• Reactivity: Chicken
• Synonyms: Total Spleen tyosine kinase; Chicken Total Spleen tyosine kinase ELISA Kit; Syk elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This Syk ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken Syk. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This Syk ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken Syk. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Syk elisa kit

Principle of the Assay: Syk ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Syk antibody and an Syk-HRP conjugate. The assay sample and buffer are incubated together with Syk-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Syk concentration since Syk from samples and Syk-HRP conjugate compete for the anti-Syk antibody binding site. Since the number of sites is limited, as more sites are occupied by Syk from the sample, fewer sites are left to bind Syk-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Syk concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Syk elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 818861
• NCBI GeneID: 20963
• Molecular Weight: 71,376 Da
• NCBI Official Full Name: SYK
• NCBI Official Synonym Full Names: spleen tyrosine kinase
• NCBI Official Symbol: Syk
• NCBI Official Synonym Symbols: Sykb
• NCBI Protein Information: tyrosine-protein kinase SYK
• UniProt Protein Name: Tyrosine-protein kinase SYK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Syk
• UniProt Synonym Gene Names: ptk72; Sykb
• UniProt Entry Name: KSYK_MOUSE

Uniprot Description

Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; Protein kinase, TK; TK group; Syk family

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; membrane; cell; cytoplasm; plasma membrane; cytoplasmic vesicle; intracellular; nucleus; B cell receptor complex

Molecular Function: protein domain specific binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; receptor signaling protein tyrosine kinase activity; protein kinase binding; protein kinase activity; integrin binding; transferase activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding; receptor binding

Biological Process: peptidyl-tyrosine phosphorylation; transcription factor import into nucleus; activation of MAPK activity; protein amino acid autophosphorylation; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of interleukin-3 biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; serotonin secretion; activation of JNK activity; leukocyte adhesion; B cell receptor signaling pathway; cell surface receptor linked signal transduction; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; angiogenesis; positive regulation of gamma-delta T cell differentiation; integrin-mediated signaling pathway; neutrophil chemotaxis; regulation of immune response; cell migration; adaptive immune response; regulation of superoxide release; immune system process; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; G-protein coupled receptor protein signaling pathway; peptidyl-serine phosphorylation; neutrophil activation during immune response; positive regulation of peptidyl-tyrosine phosphorylation; leukocyte activation during immune response; serotonin secretion by platelet; defense response to bacterium; macrophage activation during immune response; positive regulation of B cell differentiation; blood vessel morphogenesis; innate immune response; positive regulation of alpha-beta T cell proliferation; lymph vessel development; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; positive regulation of cytokine secretion