Human Coiled Coil Domain Containing Protein 80 (CCDC80) ELISA Kit | CCDC80 elisa kit

Human Coiled Coil Domain Containing Protein 80 (CCDC80) ELISA Kit

Cat. Num. AAA451700

• Gene Names: CCDC80; URB; DRO1; SSG1; okuribin
• Reactivity: Human
• Synonyms: Coiled Coil Domain Containing Protein 80 (CCDC80); Human Coiled Coil Domain Containing Protein 80 (CCDC80) ELISA Kit; DRO1; SSG1; URB; Steroid Sensitive Gene 1; Down-regulated by oncogenes protein 1; Up-regulated in BRS-3 deficient mouse homolog; CCDC80 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CCDC80. No significant cross-reactivity or interference between CCDC80 and analogues was observed.
• Sequence Length: 950

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.053ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CCDC80 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CCDC80 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCDC80 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CCDC80 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCDC80. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CCDC80. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCDC80, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CCDC80 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 41152074
• NCBI GeneID: 151887
• NCBI Accession #: NP_955805.1
• NCBI GenBank Nucleotide #: NM_199511.2
• UniProt Accession #: Q76M96; Q5PR20; Q6GPG9; Q8IVT6; Q8NBV1; Q8NHY8; D3DN67
• Molecular Weight: 10,174 Da
• NCBI Official Full Name: coiled-coil domain-containing protein 80
• NCBI Official Synonym Full Names: coiled-coil domain containing 80
• NCBI Official Symbol: CCDC80
• NCBI Official Synonym Symbols: URB; DRO1; SSG1; okuribin
• NCBI Protein Information: coiled-coil domain-containing protein 80
• UniProt Protein Name: Coiled-coil domain-containing protein 80
• Protein Family: Coiled-coil domain-containing protein
• UniProt Gene Name: CCDC80
• UniProt Synonym Gene Names: DRO1; URB

Uniprot Description

Promotes cell adhesion and matrix assembly.