Typical Standard Curve/Testing Data
Glutathione Assay Kit
EZCell Glutathione Detection Kit (Blue Fluorescence)
Synonyms
Glutathione; EZCell Glutathione Detection Kit (Blue Fluorescence); Glutathione assay kit
Application Notes
Rapid and sensitive detection of intracellular levels of GSH and activity of GST in live cells ; Screening for compounds affecting intracellular GSH levels
Preparation and Storage
Store at -20 degree C for 12 months.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for Glutathione assay kit
Background/Introduction: Glutathione (GSH) represents major low molecular-weight free thiol in living cells. As the key antioxidant in mammalian cells, intracellular GSH forms rapidly eliminated conjugates with electrophilic xenobiotics, free radicals as well as hydroperoxides. Insufficiency of cellular GSH results in oxidative stress damage and mitochondrial degeneration. Diminished levels of glutathione are symptomatic of cell stress thus; it is advantageous to monitor distribution of the intracellular GSH as an indicator of early apoptosis and cell death. EZCellTM Glutathione Detection Kit (Blue Fluorescence) utilizes Monochlorobimane (MCB), a non-fluorescent and cell-permeable dye, that forms highly fluorescent adducts with GSH (GSH-MCB) detectable at EX/EM= 380/465 +/-20 nm respectively. The reaction is catalyzed by glutathione-S-transferase (GST) and the intensity of the fluorescence reflects the amount of intracellular GSH. Hence, our kit provides a useful tool for fast and easy evaluation of the GSH effectors directly in the living cells.
Product Categories/Family for Glutathione assay kit
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Product Notes
The Glutathione (Catalog #AAA846793) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Rapid and sensitive detection of intracellular levels of GSH and activity of GST in live cells ; Screening for compounds affecting intracellular GSH levels. Researchers should empirically determine the suitability of the Glutathione for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutathione, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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