Typical Testing Data/Standard Curve (for reference only)
Glutathione S-Transferase (GST) Assay Kit | GST assay kit
CheKineTM Glutathione S-Transferase (GST) Colorimetric Assay Kit
Synonyms
Glutathione S-Transferase (GST); CheKineTM Glutathione S-Transferase (GST) Colorimetric Assay Kit; GST assay kit
Sequence Length
218
Application Notes
GST catalyzes the binding of GSH to CDNB. The conjugation is accompanied by an increase in absorbance at 340 nm. The rate of increase is directly proportional to the GST activity in the sample.
Detection Range
2-76 U/L;
Sensitivity
2 U/L
Preparation and Storage
Storage at -20 degree C and keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping: Ice packs
Shipping: Ice packs
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GST assay kit
Principle of the Assay: GST catalyzes the binding of GSH to CDNB. The conjugation is accompanied by an increase in absorbance at 340 nm. The rate of increase is directly proportional to the GST activity in the sample.
Background/Introduction: Glutathione S-transferases (GSTs) are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. The enzymes protect cells against toxicants by conjugating them to glutathione, thereby neutralizing their electrophilic sites, and rendering the products more water-soluble. The glutathione conjugatesare metabolized further to mercapturic acid and then excreted. GST has the activity of GSH-Px, also called non-Se GSH-Px, which has the function of repairing oxidative damage of macromolecule such as DNA, protein and so on. GST catalyzed reaction decreased GSH content, but did not increase GSSG content. Based on their sequence homology, substrate specificity and immunological cross-reactivity, GSTs have been grouped into species-independent classes of isozymes. These classes are comprised of both cytosolic and microsomal enzymes.
Background/Introduction: Glutathione S-transferases (GSTs) are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. The enzymes protect cells against toxicants by conjugating them to glutathione, thereby neutralizing their electrophilic sites, and rendering the products more water-soluble. The glutathione conjugatesare metabolized further to mercapturic acid and then excreted. GST has the activity of GSH-Px, also called non-Se GSH-Px, which has the function of repairing oxidative damage of macromolecule such as DNA, protein and so on. GST catalyzed reaction decreased GSH content, but did not increase GSSG content. Based on their sequence homology, substrate specificity and immunological cross-reactivity, GSTs have been grouped into species-independent classes of isozymes. These classes are comprised of both cytosolic and microsomal enzymes.
Product Categories/Family for GST assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
glutathione S-transferase, GST
Protein Family
Similar Products
Product Notes
The GST (Catalog #AAA9718984) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. GST catalyzes the binding of GSH to CDNB. The conjugation is accompanied by an increase in absorbance at 340 nm. The rate of increase is directly proportional to the GST activity in the sample. Researchers should empirically determine the suitability of the GST for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutathione S-Transferase (GST), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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