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Typical Testing Data/Standard Curve (for reference only)

Glutathione Oxidized (GSSG) Assay Kit | GSSG assay kit

CheKineTM Glutathione Oxidized (GSSG) Colorimetric Assay Kit

Synonyms
Glutathione Oxidized (GSSG); CheKineTM Glutathione Oxidized (GSSG) Colorimetric Assay Kit; GSSG assay kit
Ordering
For Research Use Only!
Application Notes
Reduced glutathione can react with DTNB and generate 2-nitro-5-mercaptobenzoic acid, which has the maximum light absorption at 412nm wavelength. The original reduced glutathione in the sample is inhibited by 2-vinylpyridine, and then GSSG is reduced to GSH by Glutathione Reductase to determine the content of GSSG.
Preparation and Storage
Storage at -20 degree C and keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping: Ice packs

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GSSG assay kit
Principle of the Assay: Reduced glutathione can react with DTNB and generate 2-nitro-5-mercaptobenzoic acid, which has the maximum light absorption at 412nm wavelength. The original reduced glutathione in the sample is inhibited by 2-vinylpyridine, and then GSSG is reduced to GSH by Glutathione Reductase to determine the content of GSSG.

Background/Introduction: Glutathione comes in two forms: reduced (GSH) and oxidized (GSSG). Glutathione oxidized is the oxidized form of Glutathione, also known as Dithioglutathione, which is formed by the oxidation of two reduced glutathione molecules. GSSG is reduced to GSH by Glutathione Reductase. Reduced glutathione exists in a large number of organisms and plays a major role. Measurement of GSH and GSSG content and GSH / GSSG ratio can reflect cells redox state, and it is also one main indicator of glutathione redox cycle.
Product Categories/Family for GSSG assay kit

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Product Notes

The GSSG (Catalog #AAA9718982) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Reduced glutathione can react with DTNB and generate 2-nitro-5-mercaptobenzoic acid, which has the maximum light absorption at 412nm wavelength. The original reduced glutathione in the sample is inhibited by 2-vinylpyridine, and then GSSG is reduced to GSH by Glutathione Reductase to determine the content of GSSG. Researchers should empirically determine the suitability of the GSSG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutathione Oxidized (GSSG), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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