Western Blot (WB)
Mouse Tubulin Monoclonal Antibody | anti-tubulin antibody
Anti-Tubulin Antibody
Applications
Western Blot, Immunofluorescence
Synonyms
Tubulin; Monoclonal Antibody; Anti-Tubulin Antibody; anti-tubulin antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
A10
Specificity
Recognize alpha-tubulin from Human, Mouse, Tetrahymena, dictyostelium, Saccharomyces.
Form/Format
Liquid
Concentration
100ug/100ul (varies by lot)
Sequence Length
423
Applicable Applications for anti-tubulin antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Western blot at dilution of 1:1000
Immunofluorescence staining at 1:100 dilutions
It is very useful as a loading control for quantitive western blotting. The antibody reconizes a 50kDa band in a Western Blot analysis.
Immunofluorescence staining at 1:100 dilutions
It is very useful as a loading control for quantitive western blotting. The antibody reconizes a 50kDa band in a Western Blot analysis.
Storage Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Immunogen
Recombinant alpha-tubulin protein from bacteria.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Western Blot (WB)
Western Blot (WB)
References
Park, IH et al. "Inhibition of hepatitis B virus replication by ligand-mediated activation of RNase L." Antiviral Res. 104:118-27 (2014). PubMed: 24509240.
Baek, KH et al. "Thrombospondin-1 mediates oncogenic Ras-induced senescence in premalignant lung tumors." J. Clin. Invest. 123:4375-89 (2013). PubMed: 24018559.
Bernasconi, R et al. "Autoadaptive ER-associated degradation defines a preemptive unfolded protein response pathway." Mol. Cell 52:783-93 (2013). PubMed: 24239290.
Reggiori, F et al. "Coronaviruses Hijack the LC3-I-positive EDEMosomes, ER-derived vesicles exporting short-lived ERAD regulators, for replication." Cell Host Microbe 7:500-8 (2010). PubMed: 20542253.
Baek, KH et al. "Thrombospondin-1 mediates oncogenic Ras-induced senescence in premalignant lung tumors." J. Clin. Invest. 123:4375-89 (2013). PubMed: 24018559.
Bernasconi, R et al. "Autoadaptive ER-associated degradation defines a preemptive unfolded protein response pathway." Mol. Cell 52:783-93 (2013). PubMed: 24239290.
Reggiori, F et al. "Coronaviruses Hijack the LC3-I-positive EDEMosomes, ER-derived vesicles exporting short-lived ERAD regulators, for replication." Cell Host Microbe 7:500-8 (2010). PubMed: 20542253.
NCBI and Uniprot Product Information
Uniprot Description
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Similar Products
Product Notes
The Tubulin tubulin (Catalog #AAA416876) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tubulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Western blot at dilution of 1:1000 Immunofluorescence staining at 1:100 dilutions It is very useful as a loading control for quantitive western blotting. The antibody reconizes a 50kDa band in a Western Blot analysis. Researchers should empirically determine the suitability of the Tubulin tubulin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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