Rabbit RAP1A Polyclonal Antibody | anti-RAP1A antibody

RAP1A Polyclonal Antibody

Cat. Num. AAA2526217

• Gene Names: RAP1A; RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: RAP1A; Polyclonal Antibody; RAP1A Polyclonal Antibody; RAP1; KREV1; KREV-1; SMGP21; anti-RAP1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1.3mg/mL (varies by lot)
• Sequence Length: 184

• Applicable Applications for anti-RAP1A antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: IHC: 1:25-1:100
• Immunogen: Recombinant protein of human RAP1A
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using RAP1A Polyclonal Antibody at dilution 1:35)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human liver cancer tissue using RAP1A Polyclonal Antibody at dilution 1:35)

Related Product Information for anti-RAP1A antibody

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 412180
• NCBI GeneID: 5906
• Molecular Weight: 20,987 Da
• NCBI Official Full Name: rap1a
• NCBI Official Synonym Full Names: RAP1A, member of RAS oncogene family
• NCBI Official Symbol: RAP1A
• NCBI Official Synonym Symbols: RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
• NCBI Protein Information: ras-related protein Rap-1A; GTP-binding protein smg p21A; Ras-related protein Krev-1
• UniProt Protein Name: Ras-related protein Rap-1A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAP1A
• UniProt Synonym Gene Names: KREV1
• UniProt Entry Name: RAP1A_HUMAN

NCBI Description

This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

RAP1A: a small G protein of the Ras family with tumor suppressor activity. Induces morphological reversion of a cell line transformed by a Ras oncogene. Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Interacts with SGSM1, SGSM2 and SGSM3.

Protein type: G protein; G protein, monomeric, Ras; Tumor suppressor; Motility/polarity/chemotaxis; G protein, monomeric

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: neuron projection; perinuclear region of cytoplasm; early endosome; late endosome; cytoplasm; plasma membrane; cytosol; cell junction

Molecular Function: GTPase activity; Rap guanyl-nucleotide exchange factor activity; protein binding; Ras GTPase binding; GTP binding; protein complex binding; protein transporter activity

Biological Process: microvillus biogenesis; platelet activation; protein transport; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of protein kinase activity; energy reserve metabolic process; blood coagulation; signal transduction; regulation of insulin secretion; Rap protein signal transduction