Typical Testing Data/Standard Curve (for reference only)
Biotin Quantitation Kit Assay Kit
Biotin Quantitation Kit (Colorimetric)
Synonyms
Biotin Quantitation Kit; Biotin Quantitation Kit (Colorimetric); Vitamin H Quantitation Kit (Colorimetric); Biotin Quantitation Kit assay kit
Application Notes
Measuring free biotin or the substitution degree of Biotinylation (biotin labels) of proteins and antibodies
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Biotin Quantitation Kit assay kit
Background/Introduction: Biotin (Vitamin H) is an important biomolecule that has been widely used in Biotinylation reactions to label antibodies or other proteins of interest. Due to its low molecular weight, biotin usually does not cause any significant changes in protein conformation and biological activity. Biotinylated proteins/antibodies are widely isolated and assayed with streptavidin pull-down assay, affinity chromatography, ELISA and Western blotting etc. offers a variety of ready-to-use Biotinylation reagents (part. # 2346, 2345, 2347, 2322, 2326, 2323). It is often desired to determine the degree of substitution of such biotinylated biomolecules. Biotin Assay Kit is based on the differential binding of Streptavidin to Biotin and a dye, 2-(4-hydroxyazobenzene) benzoic acid (HABA). When HABA is bound to Streptavidin, addition of free biotin or biotinylated biomolecules results in its displacement in the reaction. These changes are manifested in terms of decrease in the overall absorption (OD 500 nm) and can be quantitatively correlated to the amount of biotin present in solution. The kit also provides Biotin as a Standard and Biotinylated BSA as a positive control. This kit can be used to estimate as low as 60 pmol or 15 ng of biotin in solution.
Product Categories/Family for Biotin Quantitation Kit assay kit
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Product Notes
The Biotin Quantitation Kit (Catalog #AAA841690) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measuring free biotin or the substitution degree of Biotinylation (biotin labels) of proteins and antibodies. Researchers should empirically determine the suitability of the Biotin Quantitation Kit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Biotin Quantitation Kit, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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