Typical Testing Data/Standard Curve (for reference only)
EZQuant dsDNA Quantitation Kit Assay Kit
EZQuant dsDNA Quantitation Kit (Fluorometric)
Synonyms
EZQuant dsDNA Quantitation Kit; EZQuant dsDNA Quantitation Kit (Fluorometric); EZQuant dsDNA Quantitation Kit assay kit
Application Notes
Quantitation of dsDNA from viral DNA, miniprep DNA, genomic DNA, PCR amplification products etc.
Samples
dsDNA sample in solution
Detection Method
Fluorescence (Ex/Em = 480/530 nm)
Features & Benefits
Non-radioactive
Highly sensitive
Specifically detects dsDNA in the presence of ssDNA, RNA, and free nucleotides
Highly sensitive
Specifically detects dsDNA in the presence of ssDNA, RNA, and free nucleotides
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for EZQuant dsDNA Quantitation Kit assay kit
Product Information: EZQuant dsDNA Quantitation Kit (Fluorometric): Highly sensitive, Non-radioactive kit that specifically detects dsDNA in the presence of ssDNA, RNA, and free nucleotides. Detection limit: 40 pg
Background: Accurate and sensitive quantification of double stranded DNA (dsDNA) has wide applications. The major disadvantages of traditional absorbance based method for DNA quantitation include (1) lower sensitivity, (2) inability to distinguish between DNA and RNA, (3) interference due to contaminants commonly found in nucleic acid preparations and (4) relatively large contribution of nucleotides and single-stranded nucleic acid to the signal. MyBioSource's dsDNA Quantitation Kit is sensitive, non-radioactive and can specifically detect dsDNA in the presence of ssDNA, RNA, and free nucleotides. The assay is based on the fluorescent dsDNA-binding dye that specifically binds to dsDNA to generate fluorescence (Ex/Em = 480/530 nm), which enables the sensitive quantitation of small amounts of dsDNA (as low as 40 pg). This highly sensitive assay is ready as supplied for 96-well plate format and can be adapted to 384-well plate.
Summary: Detection method- Fluorescence (Ex/Em = 480/530 nm)
Application- Quantitation of dsDNA from viral DNA, miniprep DNA, genomic DNA, PCR amplification products etc.
Background: Accurate and sensitive quantification of double stranded DNA (dsDNA) has wide applications. The major disadvantages of traditional absorbance based method for DNA quantitation include (1) lower sensitivity, (2) inability to distinguish between DNA and RNA, (3) interference due to contaminants commonly found in nucleic acid preparations and (4) relatively large contribution of nucleotides and single-stranded nucleic acid to the signal. MyBioSource's dsDNA Quantitation Kit is sensitive, non-radioactive and can specifically detect dsDNA in the presence of ssDNA, RNA, and free nucleotides. The assay is based on the fluorescent dsDNA-binding dye that specifically binds to dsDNA to generate fluorescence (Ex/Em = 480/530 nm), which enables the sensitive quantitation of small amounts of dsDNA (as low as 40 pg). This highly sensitive assay is ready as supplied for 96-well plate format and can be adapted to 384-well plate.
Summary: Detection method- Fluorescence (Ex/Em = 480/530 nm)
Application- Quantitation of dsDNA from viral DNA, miniprep DNA, genomic DNA, PCR amplification products etc.
Product Categories/Family for EZQuant dsDNA Quantitation Kit assay kit
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Product Notes
The EZQuant dsDNA Quantitation Kit (Catalog #AAA841770) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Quantitation of dsDNA from viral DNA, miniprep DNA, genomic DNA, PCR amplification products etc. Researchers should empirically determine the suitability of the EZQuant dsDNA Quantitation Kit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EZQuant dsDNA Quantitation Kit, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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