Typical Testing Data/Standard Curve (for reference only)
BCA Protein Assay Kit - Reducing Agent Compatible Assay Kit
BCA Protein Assay Kit - Reducing Agent Compatible
Synonyms
BCA Protein Assay Kit - Reducing Agent Compatible; BCA Protein Assay Kit - Reducing Agent Compatible assay kit
Application Notes
Measuring total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
Samples
pure proteins, cell/tissue extracts or lysates
Detection Method
Absorbance (562 nm)
Features & Benefits
Simple & convenient.
Optimized for determining total protein content of samples containing strong reducing agents such as DTT (10 mM), TCEP (20 mM) & beta-mercaptoethanol (35 mM).
The assay is linear over a wide range of protein concentration (25-2000 mug/ml).
Optimized for determining total protein content of samples containing strong reducing agents such as DTT (10 mM), TCEP (20 mM) & beta-mercaptoethanol (35 mM).
The assay is linear over a wide range of protein concentration (25-2000 mug/ml).
Preparation and Storage
Room Temperature
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BCA Protein Assay Kit - Reducing Agent Compatible assay kit
Product Information: BCA Protein Assay Kit - Reducing Agent Compatible: MyBioSource's Reducing Agent Compatible BCA Protein Assay Kit is the only protein estimation kit on the market that is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM & beta-mercaptoethanol up to 35 mM. The widest detection range: 25-2000 mug/ml. Includes BSA. 1000 assays
Background: MyBioSource's Reducing Agent Compatible BCA Protein Assay Kit is the only protein estimation kit on the market that is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM & beta-mercaptoethanol up to 35 mM. This kit is adapted from MyBioSource's BCA Assay Kit II and is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in alkaline conditions. Reducing agents in samples interfere with BCA assay due to their ability to reduce Cu+2. MyBioSource's Reducing Agent Compatible BCA Protein Assay Kit is useful for determing protein concentrations of samples containing DTT, TCEP & beta-mercaptoethanol. The assay is linear over a wide range of protein concentration between 25-2000 mug/ml. In general, protein concentrations are calculated with reference to a commonly used protein standard. The kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.
Summary: Detection method- Absorbance (562 nm)
Reducing agent compatible
Background: MyBioSource's Reducing Agent Compatible BCA Protein Assay Kit is the only protein estimation kit on the market that is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM & beta-mercaptoethanol up to 35 mM. This kit is adapted from MyBioSource's BCA Assay Kit II and is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in alkaline conditions. Reducing agents in samples interfere with BCA assay due to their ability to reduce Cu+2. MyBioSource's Reducing Agent Compatible BCA Protein Assay Kit is useful for determing protein concentrations of samples containing DTT, TCEP & beta-mercaptoethanol. The assay is linear over a wide range of protein concentration between 25-2000 mug/ml. In general, protein concentrations are calculated with reference to a commonly used protein standard. The kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.
Summary: Detection method- Absorbance (562 nm)
Reducing agent compatible
Product Categories/Family for BCA Protein Assay Kit - Reducing Agent Compatible assay kit
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Product Notes
The BCA Protein Assay Kit - Reducing Agent Compatible (Catalog #AAA841871) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measuring total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents. Researchers should empirically determine the suitability of the BCA Protein Assay Kit - Reducing Agent Compatible for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCA Protein Assay Kit - Reducing Agent Compatible, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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